Teramoto K, Bowers J L, Khettry U, Palombo J D, Clouse M E
Department of Radiology, New England Deaconess Hospital, Boston, Massachusetts.
Transplantation. 1993 Apr;55(4):737-41. doi: 10.1097/00007890-199304000-00010.
A rat model of fatty liver transplantation has been developed to study primary nonfunction in fatty liver grafts. ACI rats were fed with a diet deficient in choline and methionine for 7, 14, 28, and 42 days. Fat content in the pretransplant livers was examined by gas chromatography and histology. The main constituent of the fatty droplets was determined to be triglyceride. The triglyceride concentration reached a maximum by day 14 and remained constant for an additional 28 days. Histology revealed an absence of necrosis in 14- and 28-day fatty livers but scattered hepatocytic necrosis and inflammation in 42-day fatty livers. After being given cold (UW stored, 4 degrees C) or warm (37 degrees C) ischemia, the fatty liver was orthotopically transplanted into normal ACI rats. The one-week survival of fatty liver grafts after 6, 12, 18, and 24 hr cold preservation was 5/5, 5/6, 3/8, 0/6 for 14-day fatty liver and 5/5, 4/6, 0/8, 0/6 for 42-day fatty livers. The survival of normal liver grafts was 5/5, 6/6, 5/9, 2/8, respectively. Increased survival rate was correlated with the absence of hepatocytic necrosis. The survival after 15 and 30 min warm ischemia prior to transplant was 5/5, 2/6 for normal liver grafts and 4/7, 0/6 for 28-day fatty liver graft, respectively. Fatty livers were less resistant to damage induced by cold or warm ischemia.
为了研究脂肪肝移植中的原发性无功能，已建立了脂肪肝移植的大鼠模型。将ACI大鼠用缺乏胆碱和蛋氨酸的饲料喂养7、14、28和42天。通过气相色谱法和组织学检查移植前肝脏中的脂肪含量。确定脂肪滴的主要成分是甘油三酯。甘油三酯浓度在第14天达到最高，并在接下来的28天保持恒定。组织学显示，14天和28天的脂肪肝中没有坏死，但42天的脂肪肝中有散在的肝细胞坏死和炎症。在给予冷（UW保存，4℃）或热（37℃）缺血后，将脂肪肝原位移植到正常ACI大鼠中。14天脂肪肝在6、12、18和24小时冷保存后的一周存活率分别为5/5、5/6、3/8、0/6，42天脂肪肝的存活率分别为5/5、4/6、0/8、0/6。正常肝脏移植的存活率分别为5/5、6/6、5/9、2/8。存活率的提高与肝细胞坏死的缺失相关。移植前15分钟和30分钟热缺血后的存活率，正常肝脏移植分别为5/5、2/6，28天脂肪肝移植分别为4/7、0/6。脂肪肝对冷或热缺血诱导的损伤的抵抗力较低。
