Lyon S G, Lasser E C, Stein R
University of California, San Diego, School of Medicine, Department of Radiology, La Jolla 92093.
Thromb Haemost. 1987 Oct 28;58(3):884-7.
A modification of an anti-Xa assay for plasma heparin has been devised using a low molecular weight dextran sulfate that competitively binds protein heparin neutralizers and displaces masked heparin. The addition of 0.12 mg dextran sulfate per ml of plasma permits heparin, neutralized by the products of platelet aggregation, to recover full functional activity against Xa. The assay will permit a more accurate assessment of both exogenous plasma heparin and endogenous heparin-like activity in blood samples collected with varying techniques. A further modification is proposed employing polybrene to neutralize the plasma heparin-like material providing a concurrent control for each sample that increases accuracy by eliminating the effect of varying AT-III levels which have anti-Xa activity.
已设计出一种用于血浆肝素的抗Xa测定法的改良方法，该方法使用低分子量硫酸葡聚糖，它能竞争性结合蛋白质类肝素中和剂并置换被掩盖的肝素。每毫升血浆添加0.12毫克硫酸葡聚糖可使被血小板聚集产物中和的肝素恢复对Xa的全部功能活性。该测定法将能更准确地评估用不同技术采集的血样中外源性血浆肝素和内源性类肝素活性。还提出了进一步的改良方法，即使用鱼精蛋白中和血浆类肝素物质，为每个样本提供同步对照，通过消除具有抗Xa活性的不同抗凝血酶III水平的影响来提高准确性。
