Vasiljevic B, Fira D, Topisirovic L
Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.
J Basic Microbiol. 1993;33(1):47-51. doi: 10.1002/jobm.3620330111.
The apramycin-resistant mutants of E. coli were isolated spontaneously. Mutations, aprA and aprB, conferring resistance to apramycin were located at 72 min on the E. coli genetic map where most of ribosomal genes were mapped. One of the mutants, carrying the aprB mutation, has an altered translational fidelity expressed as severe restriction of amber suppressor activity in vivo. The chromosome location and altered translational fidelity indicate that the apramycin-resistant phenotype could be a consequence of an alteration of the ribosomal structure.
大肠杆菌的阿普拉霉素抗性突变体是自发分离得到的。赋予对阿普拉霉素抗性的突变基因aprA和aprB位于大肠杆菌遗传图谱的72分钟处，那里定位了大多数核糖体基因。其中一个携带aprB突变的突变体具有改变的翻译保真度，表现为体内琥珀抑制子活性受到严重限制。染色体定位和改变的翻译保真度表明，阿普拉霉素抗性表型可能是核糖体结构改变的结果。
