Komori A, Yatsunami J, Suganuma M, Okabe S, Abe S, Sakai A, Sasaki K, Fujiki H
Cancer Prevention Division, National Cancer Center Research Institute, Tokyo, Japan.
Cancer Res. 1993 May 1;53(9):1982-5.
Tumor necrosis factor (TNF), a cytokine, and okadaic acid, a tumor promoter, strongly phosphorylated the same proteins, vimentin and heat shock protein 27, although their time courses were different. Human TNF-alpha at a concentration of 0.6 nM markedly stimulated transformation of BALB/3T3 cells initiated with 3-methylcholanthrene. The human TNF-alpha was about 1000 times more effective than the chemical tumor promoters, okadaic acid and 12-O-tetradecanoylphorbol-13-acetate. TNF induced growth of v-Ha-ras transfected BALB/3T3 cells (Bhas 42 cells), whereas it did not induce growth of nontransfected BALB/3T3 cells. Okadiac acid induced mouse TNF-alpha from Bhas 42 and BALB/3T3 cells. The results suggest that a chemical tumor promoter induces the secretion of TNF-alpha from various cells. The TNF then acts as an endogenous tumor promoter in vivo.
肿瘤坏死因子（TNF），一种细胞因子，和冈田酸，一种肿瘤促进剂，能强烈磷酸化相同的蛋白质，波形蛋白和热休克蛋白27，尽管它们的时间进程不同。浓度为0.6 nM的人肿瘤坏死因子-α能显著刺激由3-甲基胆蒽引发的BALB/3T3细胞的转化。人肿瘤坏死因子-α的效力比化学肿瘤促进剂冈田酸和12-O-十四酰佛波醇-13-乙酸酯高约1000倍。肿瘤坏死因子诱导v-Ha-ras转染的BALB/3T3细胞（Bhas 42细胞）生长，而不诱导未转染的BALB/3T3细胞生长。冈田酸从Bhas 42和BALB/3T3细胞中诱导小鼠肿瘤坏死因子-α。结果表明，化学肿瘤促进剂可诱导各种细胞分泌肿瘤坏死因子-α。然后肿瘤坏死因子在体内作为内源性肿瘤促进剂起作用。
