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DNA测序：由六聚体或五聚体文库组装而成的模块化引物。

DNA sequencing: modular primers assembled from a library of hexamers or pentamers.

作者信息

Kotler L E, Zevin-Sonkin D, Sobolev I A, Beskin A D, Ulanovsky L E

机构信息

Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.

出版信息

Proc Natl Acad Sci U S A. 1993 May 1;90(9):4241-5. doi: 10.1073/pnas.90.9.4241.

DOI:10.1073/pnas.90.9.4241
PMID:8483939
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC46482/
Abstract

Here we report a striking effect displayed by "modular primers," which consist of hexamer or pentamer oligonucleotide modules base-stacked to each other upon annealing to a DNA template. Such a combination of modules is found to prime DNA sequencing reactions uniquely, unlike either of the modules alone. We attribute this effect in part to the increase in the affinity of an oligonucleotide for the template in the presence of an adjacent module. All possible pentamer (or hexamer) sequences total 1024 (or 4096) samples, a manageable size for a presynthesized library. This approach can replace the synthesis of primers, which is the current bottleneck in time and cost of the primer walking sequencing, and can allow full automation of the closed cycle of walking.

摘要

在此，我们报告了“模块化引物”所展现的一种显著效应，这些引物由六聚体或五聚体寡核苷酸模块组成，在与DNA模板退火时彼此碱基堆积。发现这种模块组合能独特地引发DNA测序反应，这与单独的任何一个模块都不同。我们将这种效应部分归因于在相邻模块存在时寡核苷酸与模板亲和力的增加。所有可能的五聚体（或六聚体）序列总计1024（或4096）个样本，对于预合成文库来说规模可控。这种方法可以取代引物的合成，而引物合成是目前引物步移测序在时间和成本方面的瓶颈，并且可以实现步移闭环的完全自动化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3332/46482/2f38a6fd2f96/pnas01468-0478-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3332/46482/e33385296c38/pnas01468-0475-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3332/46482/1cf1e3b85520/pnas01468-0476-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3332/46482/03eef8d01200/pnas01468-0477-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3332/46482/2f38a6fd2f96/pnas01468-0478-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3332/46482/e33385296c38/pnas01468-0475-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3332/46482/1cf1e3b85520/pnas01468-0476-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3332/46482/03eef8d01200/pnas01468-0477-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3332/46482/2f38a6fd2f96/pnas01468-0478-a.jpg

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