联合学习增强兔海马突触体中的蛋白激酶C激活。
Associative learning potentiates protein kinase C activation in synaptosomes of the rabbit hippocampus.
作者信息
Sunayashiki-Kusuzaki K, Lester D S, Schreurs B G, Alkon D L
机构信息
Neural Systems Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
出版信息
Proc Natl Acad Sci U S A. 1993 May 1;90(9):4286-9. doi: 10.1073/pnas.90.9.4286.
Abstract
Using electrophysiological, biochemical, and autoradiographic techniques, changes in protein kinase C (PKC) activity in specific regions of the hippocampus have been previously implicated in classical conditioning of the nictitating membrane response of the rabbit. Here we report that activation of PKC is potentiated 2- to 3-fold in synaptosomes of the hippocampal CA1 and CA2 to -3 regions in rabbits that have undergone classical conditioning of the nictitating membrane response. This potentiation is apparently due to a change in the biochemical properties of PKC within the synaptosomes and is not a result of an increase in total PKC activity. This observation correlates a subcellular biochemical change with classical conditioning of a mammal.
摘要
运用电生理、生化及放射自显影技术，先前已有研究表明，兔瞬膜反应经典条件反射过程中，海马特定区域的蛋白激酶C（PKC）活性会发生变化。在此我们报告，在经历过瞬膜反应经典条件反射的兔中，海马CA1和CA2至CA3区域的突触体中PKC的激活增强了2至3倍。这种增强显然是由于突触体内PKC生化特性的改变，而非总PKC活性增加所致。这一观察结果将亚细胞生化变化与哺乳动物的经典条件反射联系了起来。
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本文引用的文献
1
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J Cell Biol. 1980 Sep;86(3):831-45. doi: 10.1083/jcb.86.3.831.
2
C-kinase activation prolongs Ca2+-dependent inactivation of K+ currents.C激酶激活可延长钾电流的钙依赖性失活。
Biochem Biophys Res Commun. 1986 Feb 13;134(3):1245-53. doi: 10.1016/0006-291x(86)90384-0.
3
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Methods Enzymol. 1986;124:349-52. doi: 10.1016/0076-6879(86)24026-4.
4
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