Pandolfi F, Paganelli R, Oliva A, Quinti I, Polidori V, Fanales-Belasio E, Guerra E, Aiuti F
Department of Allergy and Clinical Immunology, La Sapienza University of Rome, Italy.
Clin Exp Immunol. 1993 May;92(2):239-44. doi: 10.1111/j.1365-2249.1993.tb03386.x.
We have studied IL-6 gene expression and production by in vitro stimulated peripheral blood mononuclear cells (PBMC) isolated from common variable immunodeficiency (CVI) patients. A strong hybridization signal for the IL-6 probe was observed in mRNA extracted from phytohaemagglutinin (PHA)- and PHA/phorbol myristate acetate (PMA)-stimulated PBMC from most of 12 CVI patients analysed. IL-6 production by PHA-stimulated PBMC from 28 CVI patients was evaluated in ELISA and found to be significantly (P < 0.0001) higher than in normal controls. IL-6 production, however, did not correlate with the lymphocyte populations examined, nor with the absolute number of monocytes. We have also showed that IL-6 was able to increase IgM secretion by several Epstein-Barr virus (EBV)-transformed cell lines derived from both normal donors and CVI patients, but it failed to modify substantially the amounts of IgM and IgG produced in vitro by PBMC derived from CVI patients and activated with pokeweed mitogen (PWM) or anti-IgM. Our data indicate that IL-6 gene expression and production is increased in CVI, but CVI cells do not respond to IL-6 with increased production of immunoglobulin.
我们研究了从常见可变免疫缺陷（CVI）患者分离的体外刺激外周血单个核细胞（PBMC）中白细胞介素-6（IL-6）基因的表达及产生情况。在所分析的12例CVI患者中的大多数患者中，从经植物血凝素（PHA）和PHA/佛波酯肉豆蔻酸酯乙酸酯（PMA）刺激的PBMC提取的mRNA中观察到IL-6探针的强杂交信号。通过酶联免疫吸附测定（ELISA）评估了28例CVI患者经PHA刺激的PBMC产生IL-6的情况，发现其显著高于正常对照（P < 0.0001）。然而，IL-6的产生与所检测的淋巴细胞群体无关，也与单核细胞的绝对数量无关。我们还表明，IL-6能够增加来自正常供体和CVI患者的几种爱泼斯坦-巴尔病毒（EBV）转化细胞系的IgM分泌，但它未能实质性改变CVI患者来源并经商陆有丝分裂原（PWM）或抗IgM激活的PBMC体外产生的IgM和IgG量。我们的数据表明，CVI中IL-6基因表达和产生增加，但CVI细胞对IL-6无反应，不会增加免疫球蛋白的产生。
