Use of particle filters for increased practicability in sperm processing: application to the indirect immunobead test.

The use of sterile particle filters is described for the washing and incubation of living sperm before the indirect IBT. The filters greatly simplify the technique, shorten its duration, and remove all centrifugation steps that have potentially detrimental effects on the sperm. The same filters have a variety of applications and can be used whenever sperm have to be washed or incubated before biochemical tests including acrosome binding studies and flow cytometry.