噬菌体PhiX174裂解基因E在肉葡萄球菌TM300中的表达。
Expression of bacteriophage PhiX174 lysis gene E in Staphylococcus carnosus TM300.
作者信息
Halfmann G, Götz F, Lubitz W
机构信息
Institut für Mikrobiologie und Genetik, Universität Wien, Vienna, Austria.
出版信息
FEMS Microbiol Lett. 1993 Apr 1;108(2):139-43. doi: 10.1111/j.1574-6968.1993.tb06089.x.
Expression of the cloned PhiX174 gene E causes lysis of the Gram-negative bacterium Escherichia coli, which led to the proposal that a two-membrane system is necessary for the protein E lysis function. Gene E was cloned in an E. coli/Bacillus subtilis shuttle vector and expressed in the Gram-positive bacterium Staphylococcus carnosus TM300. Regulated gene E expression had a lethal effect on S. carnosus; however, no lysis was detected, lending support to the hypothesis.
克隆的噬菌体φX174基因E的表达会导致革兰氏阴性菌大肠杆菌裂解，这使得人们提出双膜系统对于蛋白E的裂解功能是必需的这一观点。基因E被克隆到大肠杆菌/枯草芽孢杆菌穿梭载体中，并在革兰氏阳性菌肉葡萄球菌TM300中表达。受调控的基因E表达对肉葡萄球菌有致死作用；然而，未检测到裂解现象，这为该假说提供了支持。
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