Dorsch M, Ashbolt N J, Cox P T, Goodman A E
University of NSW, School of Microbiology and Immunology, Kensington, Australia.
J Appl Bacteriol. 1994 Dec;77(6):722-6. doi: 10.1111/j.1365-2672.1994.tb02825.x.
Published 16S rDNA sequencing data for Aeromonas species were analysed and the validity of signature sequences derived from our investigations of these sequences was examined by sequencing the corresponding 16S rDNA regions of 67 environmental isolates from sewage effluents and receiving waters around Sydney and one clinical isolate, all previously classified as Aeromonas species. Species-specific probes for Aer. hydrophila and Aer. veronii were designed and tested in PCR assays and clearly discriminated these species from the other Aeromonas isolates as identified by 16S rDNA sequences.
分析已发表的气单胞菌属物种的16S rDNA测序数据，并通过对来自悉尼周围污水排放口和受纳水体的67株环境分离株以及1株临床分离株（所有这些菌株先前都被归类为气单胞菌属物种）的相应16S rDNA区域进行测序，检验了我们对这些序列研究得出的特征序列的有效性。设计了嗜水气单胞菌和维罗纳气单胞菌的种特异性探针，并在PCR分析中进行了测试，这些探针能将这两个种与16S rDNA序列鉴定的其他气单胞菌分离株清楚地区分开来。
