恶臭假单胞菌菌株G7和NCIB 9816 - 4中编码萘双加氧酶的基因序列。
Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4.
作者信息
Simon M J, Osslund T D, Saunders R, Ensley B D, Suggs S, Harcourt A, Suen W C, Cruden D L, Gibson D T, Zylstra G J
机构信息
Amgen Inc., Amgen Center, Thousand Oaks, CA 91320-1789.
出版信息
Gene. 1993 May 15;127(1):31-7. doi: 10.1016/0378-1119(93)90613-8.
The multicomponent enzyme, naphthalene dioxygenase, initiates the metabolism of naphthalene by Pseudomonas putida strains G7 (PpG7) and NCIB 9816-4 (Pp9816-4). The genes involved (nahAaAbAcAd) are encoded by the NAH7 and pDTG1 plasmids, respectively, and form part of the nah operon. The locations of the structural genes were determined on previously cloned fragments of DNA. The nucleotide (nt) sequences were determined for nahAaAb from Pp9816-4 and for nahAaAbAcAd from PpG7. The appropriate open reading frames were identified using N-terminal amino acid sequences determined from the purified proteins. The two nt sequences showed 93% homology, with the least homology seen upstream from the promoter region.
多组分酶萘双加氧酶启动恶臭假单胞菌菌株G7（PpG7）和NCIB 9816-4（Pp9816-4）对萘的代谢。所涉及的基因（nahAaAbAcAd）分别由NAH7和pDTG1质粒编码，并构成nah操纵子的一部分。结构基因的位置是在先前克隆的DNA片段上确定的。测定了来自Pp9816-4的nahAaAb和来自PpG7的nahAaAbAcAd的核苷酸（nt）序列。使用从纯化蛋白质中确定的N端氨基酸序列鉴定了合适的开放阅读框。这两个nt序列显示出93%的同源性，在启动子区域上游的同源性最低。
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