Lucena F, Muniesa M, Puig A, Araujo R, Jofre J
Department of Microbiology, University of Barcelona, Spain.
J Virol Methods. 1995 Aug;54(2-3):121-30. doi: 10.1016/0166-0934(95)00034-r.
A membrane of inorganic material with a honeycomb pore structure was used to concentrate phages infecting Bacteroides fragilis from drinking water. Phages were removed from the membrane with 0.25 M glycine buffer pH 9.5. Phages were not inactivated by storage in this buffer neutralized to pH 7.0 for at least 9 days at 4 degrees C. The method allows recovery of around 50% in drinking water. When the turbidity of the water increased, the efficiency of the concentration decreased markedly. The efficiency of concentration was evaluated versus a presence/absence test in 317 water samples with turbidity level below the threshold of drinking water. Results obtained by concentration of 11 provided data which were significantly more informative than the presence/absence tests carried out on 100 ml. A number of additional tests carried out with both somatic and F-specific coliphages indicated that these conclusions can be extended to these groups of bacteriophages.
使用具有蜂窝状孔结构的无机材料膜从饮用水中浓缩感染脆弱拟杆菌的噬菌体。用pH 9.5的0.25 M甘氨酸缓冲液从膜上去除噬菌体。噬菌体在4℃下于该缓冲液中储存至少9天并中和至pH 7.0后不会失活。该方法可在饮用水中回收约50%的噬菌体。当水的浊度增加时，浓缩效率显著降低。在浊度低于饮用水阈值的317个水样中，将浓缩效率与存在/不存在测试进行了比较。对11个样本进行浓缩得到的结果比在100 ml样本上进行的存在/不存在测试提供了明显更多的信息。对体细胞噬菌体和F特异性大肠杆菌噬菌体进行的一些额外测试表明，这些结论可以推广到这些噬菌体组。
