用于确定抗菌药物体外协同作用的微量稀释转移平板技术。
Microdilution transfer plate technique for determining in vitro synergy of antimicrobial agents.
作者信息
Dougherty P F, Yotter D W, Matthews T R
出版信息
Antimicrob Agents Chemother. 1977 Feb;11(2):225-8. doi: 10.1128/AAC.11.2.225.
Abstract
A microdilution transfer plate technique for determining in vitro synergy of antimicrobial agents is described. Combinations of gentamicin-nalidixic acid against Proteus mirabilis and rifampin-amphotericin B against Candida albicans are used as examples to demonstrate the technique. Results correlate with published data obtained by conventional methods. The technique is effective for evaluating the in vitro effects of antimicrobial agent combinations against both bacteria and fungi. The technique enables one to produce a checkerboard gradient in a fast, convenient, and reproducible way; results are easily visualized.
摘要
本文描述了一种用于测定抗菌药物体外协同作用的微量稀释转移平板技术。以庆大霉素-萘啶酸联合针对奇异变形杆菌以及利福平-两性霉素B联合针对白色念珠菌为例，演示了该技术。结果与通过传统方法获得的已发表数据相关。该技术对于评估抗菌药物组合对细菌和真菌的体外作用均有效。该技术能够以快速、便捷且可重复的方式产生棋盘梯度；结果易于观察。
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