Bizzaro N
Laboratory of Clinical Pathology, Ospedale Civile, S. Doná di Piave, Venice, Italy.
Arch Pathol Lab Med. 1993 May;117(5):528-30.
Nineteen cases of leukocyte agglutination were observed in the last 6 years, while performing routine blood cell counts with an autoanalyzer (Technicon H6000). The phenomenon occurred only in edetic acid-anticoagulated samples and only at room temperature. Leukocyte aggregation could be reproduced by incubating patient serum samples or edetic acid-plasma with whole blood from ABO-compatible healthy control subjects. When patient serum samples or plasma samples were first preincubated with anti-IgM serum and then mixed with normal blood, leuko-agglutinates were not observed, suggesting that a plasma factor, probably an IgM, was responsible for the phenomenon. Nonetheless, IgM presence could not be correlated with any particular disease or the use of specific drugs.
在过去6年中，使用自动分析仪（Technicon H6000）进行常规血细胞计数时，观察到19例白细胞凝集现象。该现象仅发生在乙二胺四乙酸抗凝的样本中，且仅在室温下出现。通过将患者血清样本或乙二胺四乙酸血浆与ABO血型相容的健康对照受试者的全血孵育，可以重现白细胞聚集。当患者血清样本或血浆样本先用抗IgM血清预孵育，然后与正常血液混合时，未观察到白细胞凝集，这表明一种血浆因子，可能是IgM，是该现象的原因。尽管如此，IgM的存在与任何特定疾病或特定药物的使用均无关联。
