Eicher E M, Womack J E
Biochem Genet. 1977 Feb;15(1-2):1-8. doi: 10.1007/BF00484544.
Three alleles at the Gpt-1 (glutamic-pyruvic transaminase-1) locus in the mouse, as identified by electrophoresis on cellulose acetate, and their distribution among inbred mouse strains and wild stocks are described. The Gpt-1 locus was shown to control the soluble form of the enzyme. Three-point linkage analysis established th location of Gpt-1 on chromosome 15 between uw and bt. In addition, a new staining procedure is described that allows the visualization of GPT activity on gels by the deposition of formazan. This is an improvement over previous methods that produced bands of nonfluorescence against a fluorescent background.
本文描述了通过醋酸纤维素电泳鉴定出的小鼠Gpt-1（谷氨酸丙酮酸转氨酶-1）位点的三个等位基因，以及它们在近交小鼠品系和野生种群中的分布情况。研究表明，Gpt-1位点控制着该酶的可溶性形式。三点连锁分析确定了Gpt-1在15号染色体上uw和bt之间的位置。此外，还描述了一种新的染色方法，该方法可通过甲臜沉积使凝胶上的GPT活性可视化。这是对先前方法的改进，先前方法在荧光背景下产生非荧光条带。
