Winek C L, Morris E M, Wahba W W
Allegheny County Department of Laboratories, Pittsburgh, PA 15219.
J Anal Toxicol. 1993 Mar-Apr;17(2):93-8. doi: 10.1093/jat/17.2.93.
Thirty-two New Zealand Albino rabbits (1.5-2.0 kg) were dosed on a daily basis with 20 mg/kg nortriptyline (NT) prior to feeding for a period of five days. On the fifth day of dosing, the animals were sacrificed approximately 1.5 h after the final dose. A comparison was made of nortriptyline concentrations in the blood and bone marrow at the time of sacrifice, and between bone marrow collected at the time of sacrifice and bone marrow collected at 2, 4, 6, 12, 24, 36, and 48 h after sacrifice. The results indicate that a linear relationship exists between blood and bone marrow NT concentrations, with an average marrow-to-blood ratio of 29.98 +/- 3.91 and a correlation coefficient of 0.956. Additionally, there was no significant difference (p > 0.05) observed between NT concentrations in bone marrow at the time of sacrifice and its concentration up to 24 h after sacrifice. The results indicate that bone marrow may be used to predict blood concentrations of NT up to 24 h after death when a suitable blood sample is not available.
32只新西兰白兔（体重1.5 - 2.0千克）在每日喂食前，连续五天每天给予20毫克/千克的去甲替林（NT）。在给药的第五天，最后一次给药后约1.5小时处死动物。比较处死时血液和骨髓中的去甲替林浓度，以及处死时采集的骨髓与处死2、4、6、12、24、36和48小时后采集的骨髓中的去甲替林浓度。结果表明，血液和骨髓中的NT浓度之间存在线性关系，平均骨髓与血液的比率为29.98±3.91，相关系数为0.956。此外，处死时骨髓中的NT浓度与其处死24小时内的浓度之间未观察到显著差异（p>0.05）。结果表明，当无法获得合适的血液样本时，骨髓可用于预测死亡后24小时内NT的血液浓度。
