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菠菜叶绿体的长链脂酰辅酶A合成酶活性集中在包膜中。

Long-chain acyl-coenzyme A synthetase activity of spinach chloroplasts is concentrated in the envelope.

作者信息

Roughan P G, Slack C R

出版信息

Biochem J. 1977 Feb 15;162(2):457-9. doi: 10.1042/bj1620457.

DOI:10.1042/bj1620457
PMID:849293
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164620/
Abstract

Purified chloroplasts were disrupted and then fractionated by discontinuous sucrose-density-gradient centrifugation. Envelopes contained long-chain acyl-CoA synthetase at a specific activity 80 times the activity in the lamellae or the stroma. Acetyl-CoA synthetase was concentrated in the stroma, and chlorophyll was confined to the lamellae membranes. Phospholipase D was not detected in any fraction.

摘要

纯化的叶绿体被破坏，然后通过不连续蔗糖密度梯度离心进行分级分离。包膜中长链酰基辅酶A合成酶的比活性是片层或基质中活性的80倍。乙酰辅酶A合成酶集中在基质中，叶绿素则局限于片层膜中。在任何级分中均未检测到磷脂酶D。

相似文献

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Long-chain acyl-coenzyme A synthetase activity of spinach chloroplasts is concentrated in the envelope.菠菜叶绿体的长链脂酰辅酶A合成酶活性集中在包膜中。
Biochem J. 1977 Feb 15;162(2):457-9. doi: 10.1042/bj1620457.
2
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本文引用的文献

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Quantitative analysis of brain and spinach leaf lipids employing silicic acid column chromatography and acetone for elution of glycolipids.采用硅酸柱色谱法和丙酮洗脱糖脂对脑和菠菜叶脂质进行定量分析。
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COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
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Fat metabolism in higher plants. XLVI. Nature of the substrate and the product of oleyl coenzyme A desaturase from Carthamus tinctorius.高等植物中的脂肪代谢。XLVI. 来自红花的油酰辅酶A去饱和酶的底物和产物的性质。
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A simplified method for the quantitative assay of small amounts of protein in biologic material.一种用于定量测定生物材料中少量蛋白质的简化方法。
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Rapid isolation of a plant microsomal fraction by Mg2+--precipitation.通过镁离子沉淀法快速分离植物微粒体组分
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Factors involved in fatty acyl CoA desaturation by fungal microsomes. The relative roles of acyl CoA and phospholipids as substrates.真菌微粒体中脂肪酸辅酶A去饱和作用的相关因素。酰基辅酶A和磷脂作为底物的相对作用。
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