Rönnemaa T, Doherty N S
Atherosclerosis. 1977 Mar;26(3):261-72. doi: 10.1016/0021-9150(77)90079-x.
Rats were made hypercholesterolemic by feeding them a high-cholesterol, olive oil diet for one week. The effect of sera and 35,000 X g supernatants of liver homogenates on collagen synthesis was studied in isolated aortas, cultured arterial smooth muscle cells and the same cells in suspension. Compared to the preparations from normal rats, the liver preparations from hyperlipidemic rats stimulated collagen synthesis in both isolated aortas and cultured smooth muscle cells by about 25%. In these test systems hyperlipidemic serum was without effect but when added to smooth muscle cells incubated in suspension, produced a significant increase in the amount of collagen secreted. Hyperlipidemic serum caused an increase of about 50% in the incorporation of [3H]-thymidine by cultured smooth muscle cells.
通过给大鼠喂食高胆固醇橄榄油饮食一周，使其形成高胆固醇血症。研究了血清和肝脏匀浆35,000×g上清液对离体主动脉、培养的动脉平滑肌细胞以及悬浮状态下的相同细胞中胶原蛋白合成的影响。与正常大鼠的制剂相比，高脂血症大鼠的肝脏制剂使离体主动脉和培养的平滑肌细胞中的胶原蛋白合成增加了约25%。在这些测试系统中，高脂血症血清没有作用，但当添加到悬浮培养的平滑肌细胞中时，会使分泌的胶原蛋白量显著增加。高脂血症血清使培养的平滑肌细胞中[3H] - 胸腺嘧啶核苷的掺入量增加了约50%。
