Rönnemaa T, Pelliniemi T T, Kulonen E
Atherosclerosis. 1976 Jul-Aug;24(1-2):311-21. doi: 10.1016/0021-9150(76)90086-1.
Hypercholesterolemia was induced in rats by feeding them a high cholesterol olive oil diet. The livers were homogenized in modified Krebs-Ringer medium and centrifuged at 35,000 x g. The supernatants from livers of both hypercholesterolemic and normal rats were found to stimulate collagen synthesis in freshly isolated embryonic chick-tendon fibroblasts. However, this was significantly greater in the supernatants from fatty livers. The stimulating principle proceed to be dialyzable, non-lipid and heat-stable. There were at least two factors involved, the more effective of which was trypsin-sensitive, with a molecular weight below 2,000. The results suggest that a mediator is formed in the livers of hypercholesterolemic rats which might be responsible for the enhanced collagen synthesis of fibrotic processes vivo, e.g., in atherosclerosis and liver cirrhosis.
通过给大鼠喂食高胆固醇橄榄油饮食诱导其发生高胆固醇血症。将肝脏在改良的克雷布斯-林格培养基中匀浆，并以35,000×g离心。发现高胆固醇血症大鼠和正常大鼠肝脏的上清液均能刺激新鲜分离的胚胎鸡肌腱成纤维细胞中的胶原蛋白合成。然而，脂肪肝上清液中的这种刺激作用明显更强。刺激因子可透析、非脂质且热稳定。至少涉及两个因素，其中更有效的因素对胰蛋白酶敏感，分子量低于2000。结果表明，在高胆固醇血症大鼠的肝脏中形成了一种介质，它可能是体内纤维化过程（如动脉粥样硬化和肝硬化）中胶原蛋白合成增强的原因。
