干燥过程中角膜基质中胶原纤维的结构转变。一项X射线散射研究。
Structural transformation of collagen fibrils in corneal stroma during drying. An x-ray scattering study.
作者信息
Fratzl P, Daxer A
机构信息
Institut für Festkörperphysik der Universität Wien, Vienna, Austria.
出版信息
Biophys J. 1993 Apr;64(4):1210-4. doi: 10.1016/S0006-3495(93)81487-5.
Abstract
X-ray scattering experiments were performed on human corneas during drying. In a first stage the collagen interfibrillar distance decreased considerably. Then, at a critical point of dehydration, a structural transformation of the collagen fibrils was observed. This finding leads to a two-stage drying model, which explains the discrepancy between the collagen fibril diameters determined by x-ray scattering and by electron microscopy. Our results strongly suggest that the collagen fibrils in the corneal stroma are surrounded by a cylindrical coating made mainly of proteoglycans. The coating appears as a three-dimensional fractal network with fractal dimension of 2.7 +/- 0.1.
摘要
在干燥过程中对人角膜进行了X射线散射实验。在第一阶段，胶原纤维间距离显著减小。然后，在脱水的临界点，观察到胶原纤维的结构转变。这一发现导致了一个两阶段干燥模型，该模型解释了通过X射线散射和电子显微镜测定的胶原纤维直径之间的差异。我们的结果强烈表明，角膜基质中的胶原纤维被主要由蛋白聚糖组成的圆柱形涂层所包围。该涂层呈现为分形维数为2.7±0.1的三维分形网络。
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本文引用的文献
1
The structure and transparency of the cornea.角膜的结构与透明度。
J Physiol. 1957 Apr 30;136(2):263-86. doi: 10.1113/jphysiol.1957.sp005758.
2
Collagen packing and mineralization. An x-ray scattering investigation of turkey leg tendon.胶原蛋白的堆积与矿化。火鸡腿肌腱的X射线散射研究。
Biophys J. 1993 Jan;64(1):260-6. doi: 10.1016/S0006-3495(93)81362-6.
3
Collagen fibrils of the vertebrate corneal stroma.脊椎动物角膜基质的胶原纤维。
J Ultrastruct Res. 1981 Feb;74(2):232-9. doi: 10.1016/s0022-5320(81)80081-0.
4
Synchrotron x-ray diffraction study of corneal stroma.
J Mol Biol. 1982 Oct 5;160(4):593-607. doi: 10.1016/0022-2836(82)90317-5.
5
Neutron diffraction studies of the corneal stroma.角膜基质的中子衍射研究。
J Mol Biol. 1982 Mar 5;155(3):389-93. doi: 10.1016/0022-2836(82)90011-0.
6
Light scattering in the cornea.角膜中的光散射。
J Opt Soc Am. 1969 Jun;59(6):766-74. doi: 10.1364/josa.59.000766.
7
On the transparency of the stroma in the mammalian cornea.关于哺乳动物角膜基质的透明度
IEEE Trans Biomed Eng. 1970 Jul;17(3):186-90. doi: 10.1109/tbme.1970.4502732.
8
Wave-length dependencies of light scattering in normal and cold swollen rabbit corneas and their structural implications.正常及冷肿胀兔角膜中光散射的波长依赖性及其结构意义。
J Physiol. 1973 Sep;233(3):589-612. doi: 10.1113/jphysiol.1973.sp010325.
9
Axially projected collagen structures.轴向投影的胶原结构。
Proc R Soc Lond B Biol Sci. 1974 Aug 27;187(1086):37-46. doi: 10.1098/rspb.1974.0059.
10
Preservation of corneal collagen fibril structure using low-temperature procedures for electron microscopy.使用低温程序进行电子显微镜检查以保存角膜胶原纤维结构。
J Ultrastruct Mol Struct Res. 1986 Jul-Sep;96(1-3):172-5. doi: 10.1016/0889-1605(86)90018-2.
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