Launois P, Vandenbussche P, M'Bayame N N, Drowart A, Van Vooren J P, Sarthou J L, Millan J, Huygen K
Institut Pasteur de Dakar, Service Immunologie, Sénégal.
Cell Immunol. 1993 May;148(2):283-90. doi: 10.1006/cimm.1993.1112.
IL-6 production was examined in PBMC cultures from healthy leprosy contacts and from leprosy patients stimulated with the purified mycobacterial 18-, 65-, and 70-kDa heat-shock proteins (hsp) and the secreted fibronectin-binding antigen 85 (Ag85). In lepromin-negative contacts, the 70-kDa hsp was the only antigen capable of eliciting significant IL-6 production. In lepromin-positive contacts, Ag85, the 65- and the 70-kDa hsp induced substantial IL-6 titers. IL-6 levels induced with the 70-kDa antigen were about fourfold higher than with the 65-kDa hsp or with Ag85. The 18-kDa antigen did not induce any IL-6 in these healthy contacts. PBMC from tuberculoid leprosy patients produced even more elevated levels of IL-6, and PBMC from lepromatous leprosy patients produced extremely high levels of IL-6. All antigens were capable of inducing IL-6 in leprosy patients. Highest levels were found in cultures stimulated with the 65-kDa hsp, and lowest levels were in cultures stimulated with the 18-kDa hsp.
在来自健康麻风接触者以及麻风患者的外周血单核细胞（PBMC）培养物中检测了白细胞介素-6（IL-6）的产生情况，这些培养物用纯化的分枝杆菌18 kDa、65 kDa和70 kDa热休克蛋白（hsp）以及分泌型纤连蛋白结合抗原85（Ag85）进行刺激。在麻风菌素阴性的接触者中，70 kDa hsp是唯一能够引发显著IL-6产生的抗原。在麻风菌素阳性的接触者中，Ag85、65 kDa和70 kDa hsp诱导出大量的IL-6滴度。用70 kDa抗原诱导的IL-6水平比用65 kDa hsp或Ag85诱导的水平高约四倍。18 kDa抗原在这些健康接触者中未诱导出任何IL-6。结核样型麻风患者的PBMC产生的IL-6水平更高，瘤型麻风患者的PBMC产生的IL-6水平极高。所有抗原都能够在麻风患者中诱导IL-6产生。在用65 kDa hsp刺激的培养物中发现的水平最高，在用18 kDa hsp刺激的培养物中水平最低。
