来自小鼠浆细胞瘤MPC 11的内池A 型颗粒中的DNA聚合酶
DNA polymerases from intracisternal A-type particles on the mouse plasmacytoma MPC 11.
作者信息
Schmidt J, Pragnell I B, Weimann B J
出版信息
Eur J Biochem. 1977 Mar 1;73(2):493-7. doi: 10.1111/j.1432-1033.1977.tb11342.x.
Abstract
Intracisternal A particles from mouse plasma cell tumors were isolated from microsomal vesiclels by detergent treatment and separated in linear sucrose gradients. Four peaks of DNA polymerase activities banding at densities of 1.30, 1.24, 1.20--1.22 and 1.13 g/cm3 were observed assaying on poly(rA).oligo(dT). Solubolized DNA polymerases of the 1.30 g/cm3...
摘要
通过去污剂处理从小鼠浆细胞瘤的脑池内A颗粒中分离出微粒体囊泡，并在线性蔗糖梯度中进行分离。在聚（rA）·寡聚（dT）上进行测定时，观察到DNA聚合酶活性的四个峰，其密度分别为1.30、1.24、1.20 - 1.22和1.13 g/cm³。密度为1.30 g/cm³的可溶DNA聚合酶……
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