细胞质颗粒与氨酰转移酶I活性
Cytoplasmic particles and aminoacyl transferase I activity.
作者信息
Shelton E, Kuff E L, Maxwell E S, Harrington J T
出版信息
J Cell Biol. 1970 Apr;45(1):1-8. doi: 10.1083/jcb.45.1.1.
Abstract
It has been possible to show by electron microscopy of samples selected from sucrose gradients that particles of specific size and shape are present in supernatant fluids derived from nucleated animal and plant cells, but not in extracts from Escherichia coli. Aminoacyl transferase I activity in these same gradients sediments in two peaks representing material of approximately 5-7S and 18-20S. A rectangular particle, 100 x 145 A in size, sediments at 19S and coincides with the second peak of transferase I activity. The possibility that the rectangular particle may be a "carrier" particle associated with transferase I is discussed.
摘要
通过对从蔗糖梯度中选取的样本进行电子显微镜观察，已证实来自有核动植物细胞的上清液中存在特定大小和形状的颗粒，而大肠杆菌提取物中则没有。在这些相同的梯度中，氨酰转移酶I活性以两个峰沉降，分别代表约5 - 7S和18 - 20S的物质。一种大小为100×145埃的矩形颗粒在19S沉降，与转移酶I活性的第二个峰重合。文中讨论了这种矩形颗粒可能是与转移酶I相关的“载体”颗粒的可能性。
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