Winslow R M, Swenberg M L, Berger R L, Shrager R I, Luzzana M, Samaja M, Rossi-Bernardi L
J Biol Chem. 1977 Apr 10;252(7):2331-7.
Oxygen equilibrium curves of fresh, normal human blood have been measured by new methods which allow the control of pH, pCO2, and 2,3-diphosphoglycerate and which yield higher accuracy at the extremes of saturation than was possible previously. The curve determined by these techniques lies slightly to the right of the standard curve of Roughton et al. (Roughton, F.J.W., Deland, E.C., Kernohan, J.C., and Severinghaus, J.W. (1972) in Oxygen Affinity of Hemoglobin and Red Cell Acid Base Status (Astrup, P., and Rørth, M., eds) pp. 73-83, Academic Press, New York). The greatest difference is at low oxygen saturation, probably owing to the fact that the latter data were obtained under conditions which would lead to depletion of cellular 2,3-diphosphoglycerate. The range of p50 (oxygen pressure at half-saturation) values for four normal subjects was 28.3 mm Hg to 29.0 mm Hg. Adair's stepwise oxygenation scheme has been used to analyze the curves with the result that a1 = 0.1514 X 10(-1) (+/- 10%) mm-1; a2 = 0.9723 X 10(-3) (+/- 8%) mm-2; a3 = 0.1703 X 10(-3) (+/- 50%) mm-3; a4 = 0.1671 X 10(-5) (+/- 2%) mm-4 for the best of four data sets. Because these constants are very sensitive to changes in the shape of the oxygenation curve, this analysis is much more useful than p50 measurements in the investigation of the various allosteric effectors of the function of hemoglobin within the red cell.
采用新方法测量了新鲜正常人血液的氧平衡曲线，该方法能够控制pH值、二氧化碳分压（pCO₂）和2,3 - 二磷酸甘油酸（2,3-DPG），并且在饱和度极值处比以往方法具有更高的准确性。通过这些技术测定的曲线略位于Roughton等人标准曲线的右侧（Roughton, F.J.W., Deland, E.C., Kernohan, J.C., and Severinghaus, J.W. (1972) 载于《血红蛋白的氧亲和力与红细胞酸碱状态》（Astrup, P., and Rørth, M., 编），第73 - 83页，学术出版社，纽约）。最大差异出现在低氧饱和度时，这可能是因为后者的数据是在会导致细胞内2,3 - DPG耗尽的条件下获得的。四名正常受试者的半数饱和氧分压（p50）值范围为28.3毫米汞柱至29.0毫米汞柱。采用Adair逐步氧合方案对这些曲线进行分析，结果是在四个数据集中最佳的一组数据中，a1 = 0.1514×10⁻¹（±10%）毫米⁻¹；a2 = 0.9723×10⁻³（±8%）毫米⁻²；a3 = 0.1703×10⁻³（±50%）毫米⁻³；a4 = 0.1671×10⁻⁵（±2%）毫米⁻⁴。由于这些常数对氧合曲线形状的变化非常敏感，因此在研究红细胞内血红蛋白功能的各种变构效应剂时，这种分析比p50测量更有用。
