Zapata-Sirvent R L, Hansbrough J F
Department of Surgery University of California, San Diego Medical Center 92103.
J Burn Care Rehabil. 1993 Mar-Apr;14(2 Pt 1):132-40. doi: 10.1097/00004630-199303000-00002.
We tested two topical antimicrobial agents (TAAs), silver sulfadiazine and mafenide acetate, to determine their cytotoxic effects when human lymphocytes and neutrophils were incubated with the agents in vitro for 30 minutes. Dilute concentrations of both TAAs markedly inhibited neutrophil respiratory burst activity and mitogen-stimulated lymphocyte proliferation (p < 0.05). The components of silver sulfadiazine (silver and sulfadiazine) were separately tested, and each component inhibited both neutrophil and lymphocyte functions. Mafenide acetate markedly decreased intracellular Ca+2 flux in lymphocytes. The effects of the TAAs were partially reversed when cells were washed and resuspended in medium after they were exposed in vitro to the TAAs. Commonly used TAAs may contribute to local immune dysfunction in the patient with burns. Because evidence suggests that T lymphocytes may participate in wound healing, prolonged treatment with TAAs may also effect certain aspects of wound healing.
我们测试了两种局部抗菌剂（TAAs），即磺胺嘧啶银和醋酸甲磺灭脓，以确定当人淋巴细胞和中性粒细胞在体外与这些药剂孵育30分钟时它们的细胞毒性作用。两种TAAs的稀释浓度均显著抑制中性粒细胞呼吸爆发活性和丝裂原刺激的淋巴细胞增殖（p<0.05）。分别测试了磺胺嘧啶银的成分（银和磺胺嘧啶），每种成分均抑制中性粒细胞和淋巴细胞功能。醋酸甲磺灭脓显著降低淋巴细胞内Ca+2通量。当细胞在体外暴露于TAAs后洗涤并重悬于培养基中时，TAAs的作用部分逆转。常用的TAAs可能导致烧伤患者局部免疫功能障碍。因为有证据表明T淋巴细胞可能参与伤口愈合，TAAs的长期治疗也可能影响伤口愈合的某些方面。
