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在鼻咽抽吸物中检测呼吸道合胞病毒时，VIDAS呼吸道合胞病毒检测法、雅培呼吸道合胞病毒检测试剂盒与直接免疫荧光法的比较。

Comparison of the VIDAS RSV assay and the Abbott Testpack RSV with direct immunofluorescence for detection of respiratory syncytial virus in nasopharyngeal aspirates.

作者信息

Miller H, Milk R, Diaz-Mitoma F

机构信息

Regional Virology Laboratory, Children's Hospital of Eastern Ontario, Ottawa, Canada.

出版信息

J Clin Microbiol. 1993 May;31(5):1336-8. doi: 10.1128/jcm.31.5.1336-1338.1993.

DOI:10.1128/jcm.31.5.1336-1338.1993
PMID:8501239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC262932/
Abstract

The sensitivity and accuracy of the VIDAS RSV assay in testing fresh specimens were 82.7 and 87.1%, respectively, whereas specimens previously frozen at -70 degrees C gave a sensitivity of 96.2% and an accuracy of 95.4%. The sensitivity and accuracy of Abbott Testpack RSV were 92.6 and 91.3% for fresh specimens and 86.8 and 88.1% for frozen specimens. The advantages and drawbacks of the two assays are discussed.

摘要

VIDAS呼吸道合胞病毒检测法检测新鲜标本时的灵敏度和准确度分别为82.7%和87.1%，而之前在-70摄氏度冷冻的标本，其灵敏度为96.2%，准确度为95.4%。雅培呼吸道合胞病毒检测试剂盒检测新鲜标本时的灵敏度和准确度分别为92.6%和91.3%，检测冷冻标本时的灵敏度和准确度分别为86.8%和88.1%。文中讨论了这两种检测法的优缺点。

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本文引用的文献

1
Reduction of nonspecific fluorescence in respiratory specimens by pretreatment with N-acetylcysteine.通过用N-乙酰半胱氨酸预处理降低呼吸道标本中的非特异性荧光。
J Clin Microbiol. 1986 Sep;24(3):470-1. doi: 10.1128/jcm.24.3.470-471.1986.
2
Rapid detection of respiratory syncytial virus by a biotin-enhanced immunoassay: test performance by laboratory technologists and housestaff.通过生物素增强免疫测定法快速检测呼吸道合胞病毒：实验室技术人员和住院医师的检测性能
Pediatr Infect Dis J. 1989 Dec;8(12):865-9. doi: 10.1097/00006454-198912000-00008.
3
Rapid detection of influenza virus infections in human fetal lung diploid cell cultures.人胚肺二倍体细胞培养物中流感病毒感染的快速检测
J Infect. 1989 May;18(3):269-78. doi: 10.1016/s0163-4453(89)80063-5.
4
Evaluation of five methods for respiratory syncytial virus detection.五种呼吸道合胞病毒检测方法的评估
J Clin Microbiol. 1990 May;28(5):1021-5. doi: 10.1128/jcm.28.5.1021-1025.1990.