人喉表皮样癌细胞（HEp-2）细胞培养与雅培呼吸道合胞病毒酶免疫测定的比较。
Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay.
作者信息
Bromberg K, Tannis G, Daidone B, Clarke L, Sierra M
出版信息
J Clin Microbiol. 1987 Feb;25(2):434-6. doi: 10.1128/jcm.25.2.434-436.1987.
Abstract
Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection in children. Rapid identification of RSV infections would allow for specific chemotherapy. We evaluated a means of rapid diagnosis, the Abbott enzyme immunoassay (EIA), by using 314 stored nasopharyngeal aspirates. RSV antigens were identified in 62 of 66 RSV culture-positive specimens. An additional 37 specimens from which RSV was not isolated were positive in the EIA. Of these, 29 were confirmed as truly positive by a blocking assay, for a total of 95 (66 + 29) positive specimens. The sensitivity of the EIA for total positive samples was 96% (91/95) versus 69% (66/95) for cell culture. The specificity of the EIA was 96% (211/219). In these stored specimens, Abbott EIA was superior to cell culture for the detection of RSV.
摘要
呼吸道合胞病毒（RSV）是儿童下呼吸道感染的主要病因。快速识别RSV感染将有助于进行特异性化疗。我们通过使用314份储存的鼻咽抽吸物评估了一种快速诊断方法，即雅培酶免疫测定法（EIA）。在66份RSV培养阳性标本中的62份中鉴定出了RSV抗原。另外37份未分离出RSV的标本在EIA中呈阳性。其中，29份通过阻断试验确认为真正阳性，总计95份（66 + 29）阳性标本。EIA对总阳性样本的敏感性为96%（91/95），而细胞培养为69%（66/95）。EIA的特异性为96%（211/219）。在这些储存的标本中，雅培EIA在检测RSV方面优于细胞培养。
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