Thomas E E, Book L E
Department of Pathology, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
J Clin Microbiol. 1991 Mar;29(3):632-5. doi: 10.1128/jcm.29.3.632-635.1991.
The ability of two commercial immunoassays to detect respiratory syncytial virus (RSV) in respiratory specimens was evaluated as follows: 152 specimens were tested by TestPack RSV (Abbott), and 72 were tested by Ortho RSV ELISA (Ortho). Test outcomes were compared with those of virus isolation alone, direct immunofluorescence assay (DFA) alone, or virus isolation and/or DFA. TestPack RSV versus virus isolation showed 91% sensitivity, 96% specificity, 93% positive predictive value (PPV), and 95% negative predictive value (NPV). TestPack RSV versus DFA showed 89% sensitivity, 97% specificity, 96% PPV, and 93% NPV. When TestPack RSV performance was compared with that of virus isolation and DFA, the sensitivity was 87% and the specificity was 100%. Ortho RSV ELISA versus virus isolation showed 88% sensitivity, 87% specificity, 79% PPV, and 93% NPV. Ortho RSV ELISA versus DFA showed 91% sensitivity, 88% specificity, 81% PPV and 95% NPV. When Ortho RSV ELISA performance was compared with that of virus isolation and DFA, the sensitivity was 86%, the specificity was 89%, the PPV was 86%, and the NPV was 89%. The accuracy of the TestPack RSV in combination with ease of performance and no need for specialized equipment or special skills make it an attractive alternative to DFA for rapid direct detection of RSV.
对两种商用免疫测定法检测呼吸道标本中呼吸道合胞病毒（RSV）的能力进行了如下评估：152份标本采用TestPack RSV（雅培公司）检测，72份标本采用奥索RSV酶联免疫吸附测定法（奥索公司）检测。将检测结果与单独进行病毒分离、直接免疫荧光测定法（DFA）或病毒分离和/或DFA的结果进行比较。TestPack RSV与病毒分离相比，敏感性为91%，特异性为96%，阳性预测值（PPV）为93%，阴性预测值（NPV）为95%。TestPack RSV与DFA相比，敏感性为89%，特异性为97%，PPV为96%，NPV为93%。当将TestPack RSV的性能与病毒分离和DFA的性能进行比较时，敏感性为87%，特异性为100%。奥索RSV酶联免疫吸附测定法与病毒分离相比，敏感性为88%，特异性为87%，PPV为79%，NPV为93%。奥索RSV酶联免疫吸附测定法与DFA相比，敏感性为91%，特异性为88%，PPV为81%，NPV为95%。当将奥索RSV酶联免疫吸附测定法的性能与病毒分离和DFA的性能进行比较时，敏感性为86%，特异性为89%，PPV为86%，NPV为89%。TestPack RSV的准确性、操作简便以及无需专门设备或特殊技能，使其成为DFA快速直接检测RSV的一种有吸引力的替代方法。
