Gorman S P, Mawhinney W M, Adair C G, Issouckis M
School of Pharmacy, Queen's University, Belfast.
J Med Microbiol. 1993 Jun;38(6):411-7. doi: 10.1099/00222615-38-6-411.
Surface topography of used (in situ > 12 months) and unused CAPD catheters was studied by scanning electronmicroscopy (SEM) and confocal laser scanning microscopy (CLSM). Microbial biofilm was observed on all used catheters. Disruption and removal of the attached biofilm revealed extensive pitting of the catheter surface and scoring within the catheter pores. Similar, though less extensive, surface defects were present in unused catheters. Examination by CLSM, with software specific to the determination of surface topography, showed used catheters to have a surface microrugosity greater than that of unused catheters (p < 0.0005). Adherence studies with radiolabelled Staphylococcus epidermidis demonstrated increased adherence to used than to unused catheters (p < 0.0005) after 48 h. However, when catheters were pre-treated with spent dialysate there was a substantial reduction in bacterial adherence to either catheter and no significant difference in adherence to used and unused catheters. Surface microrugosity of CAPD catheters increases during use but is unlikely to be an important factor in bacterial adherence in vivo.
通过扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）研究了使用过的（原位使用超过12个月）和未使用的持续性非卧床腹膜透析（CAPD）导管的表面形貌。在所有使用过的导管上均观察到微生物生物膜。附着生物膜的破坏和去除显示导管表面有广泛的麻点以及导管孔隙内的刻痕。未使用的导管也存在类似但程度较轻的表面缺陷。使用特定于表面形貌测定的软件进行CLSM检查显示，使用过的导管表面微粗糙度大于未使用的导管（p < 0.0005）。对放射性标记的表皮葡萄球菌进行的黏附研究表明，48小时后，其在使用过的导管上的黏附比在未使用的导管上增加（p < 0.0005）。然而，当导管用用过的透析液预处理后，细菌在两种导管上的黏附均大幅减少，且在使用过的和未使用的导管上的黏附没有显著差异。CAPD导管的表面微粗糙度在使用过程中增加，但不太可能是体内细菌黏附的重要因素。
