Budkowska A, Quan C, Groh F, Bedossa P, Dubreuil P, Bouvet J P, Pillot J
Microbial Immunology Unit, Pasteur Institute, Paris, France.
J Virol. 1993 Jul;67(7):4316-22. doi: 10.1128/JVI.67.7.4316-4322.1993.
A hepatitis B virus (HBV) binding factor (HBV-BF) was identified in normal human serum interacting with the pre-S1 and pre-S2 epitopes of the viral envelope located within the protein domains involved in recognition of hepatocyte receptor(s). This molecule was characterized as a 50-kDa glycoprotein showing an isoelectric point of 7.13 with a biological activity depending on its native molecular conformation and on intact sulfhydryl bonds. Monoclonal antibodies to HBV-BF recognized a membrane component of the normal human liver whereas they were unreactive with hepatocyte membranes of other species and with those of the HepG2 cell line. These results suggest that the HBV-BF represents a soluble fragment of the membrane component and can be related to the HBV receptor mediating attachment of HBV to human liver cells.
在正常人血清中鉴定出一种乙肝病毒（HBV）结合因子（HBV - BF），它与病毒包膜的前S1和前S2表位相互作用，这些表位位于参与识别肝细胞受体的蛋白质结构域内。该分子被鉴定为一种50 kDa的糖蛋白，其等电点为7.13，其生物活性取决于其天然分子构象和完整的巯基键。针对HBV - BF的单克隆抗体识别正常人肝脏的一种膜成分，而与其他物种的肝细胞膜以及HepG2细胞系的肝细胞膜无反应。这些结果表明，HBV - BF代表膜成分的可溶性片段，可能与介导HBV附着于人类肝细胞的HBV受体有关。
