Ammon H P, Safayhi H, Mack T, Sabieraj J
Department of Pharmacology, Eberhard-Karls University, Tübingen, FRG.
J Ethnopharmacol. 1993 Mar;38(2-3):113-9. doi: 10.1016/0378-8741(93)90005-p.
Curcumine from Curcuma longa and the gum resin of Boswellia serrata, which were demonstrated to act as anti-inflammatories in in vivo animal models, were studied in a set of in vitro experiments in order to elucidate the mechanism of their beneficial effects. Curcumine inhibited the 5-lipoxygenase activity in rat peritoneal neutrophils as well as the 12-lipoxygenase and the cyclooxygenase activities in human platelets. In a cell free peroxidation system curcumine exerted strong antioxidative activity. Thus, its effects on the dioxygenases are probably due to its reducing capacity. Boswellic acids were isolated from the gum resin of Boswellia serrata and identified as the active principles. Boswellic acids inhibited the leukotriene synthesis via 5-lipoxygenase, but did not affect the 12-lipoxygenase and the cyclooxygenase activities. Additionally, boswellic acids did not impair the peroxidation of arachidonic acid by iron and ascorbate. The data suggest that boswellic acids are specific, non-redox inhibitors of leukotriene synthesis either interacting directly with 5-lipoxygenase or blocking its translocation.
姜黄中的姜黄素以及乳香的树胶脂，在体内动物模型中已被证明具有抗炎作用，为阐明其有益作用机制，在一系列体外实验中对它们进行了研究。姜黄素抑制大鼠腹腔中性粒细胞中的5-脂氧合酶活性以及人血小板中的12-脂氧合酶和环氧化酶活性。在无细胞过氧化系统中，姜黄素表现出很强的抗氧化活性。因此，其对双加氧酶的作用可能归因于其还原能力。从乳香的树胶脂中分离出乳香酸并确定为活性成分。乳香酸通过5-脂氧合酶抑制白三烯合成，但不影响12-脂氧合酶和环氧化酶活性。此外，乳香酸不损害铁和抗坏血酸介导的花生四烯酸过氧化。数据表明，乳香酸是白三烯合成的特异性、非氧化还原抑制剂，要么直接与5-脂氧合酶相互作用，要么阻断其易位。
