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Confirmatory testing of Chlamydia trachomatis Syva enzyme immunoassay gray zone specimens by Syva direct fluorescent antibody test.

作者信息

Beebe J L, Rau M P, Albrecht K D

机构信息

Division of Laboratories, Colorado Department of Health, Denver 80220.

出版信息

Sex Transm Dis. 1993 May-Jun;20(3):140-2. doi: 10.1097/00007435-199305000-00004.

DOI:10.1097/00007435-199305000-00004
PMID:8511707
Abstract

A series of gray zone specimens with sample to cutoff ratio of 0.7 to 0.99 encountered in routine use of the Syva Microtrak (Syva, Inc.) Chlamydia trachomatis enzyme immunoassay (EIA) test for urogenital specimens were subjected to repeat testing in duplicate and high-speed centrifugation with direct fluorescent antibody testing of the centrifugate. Immunofluorescent C. trachomatis elementary bodies were observed in high-speed centrifugates in more than 40% of two series of gray zone specimens examined indicating that a C. trachomatis gray zone result would require confirmatory testing.

摘要

相似文献

1
Confirmatory testing of Chlamydia trachomatis Syva enzyme immunoassay gray zone specimens by Syva direct fluorescent antibody test.
Sex Transm Dis. 1993 May-Jun;20(3):140-2. doi: 10.1097/00007435-199305000-00004.
2
Use of PCR and direct immunofluorescence microscopy for confirmation of results obtained by Syva MicroTrak Chlamydia enzyme immunoassay.使用聚合酶链反应（PCR）和直接免疫荧光显微镜检查来确认由Syva MicroTrak衣原体酶免疫测定法获得的结果。
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J Clin Microbiol. 1994 Sep;32(9):2208-11. doi: 10.1128/jcm.32.9.2208-2211.1994.
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Comparison of the Syva Microtrak enzyme immunoassay and Abbott Chlamydiazyme in the detection of chlamydial infections in women.Syva Microtrak酶免疫测定法与雅培衣原体酶免疫测定法在检测女性衣原体感染中的比较。
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Head-to-head evaluation of five chlamydia tests relative to a quality-assured culture standard.将五种衣原体检测方法与质量保证的培养标准进行直接比较评估。
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引用本文的文献

1
Lowering the cut off value of an automated chlamydia enzyme immunoassay and confirmation by PCR and direct immunofluorescent antibody test.降低衣原体自动化酶免疫测定的临界值，并通过聚合酶链反应和直接免疫荧光抗体试验进行确认。
J Clin Pathol. 1997 Aug;50(8):681-5. doi: 10.1136/jcp.50.8.681.
2
Evaluation of the Vidas Chlamydia test to detect and verify Chlamydia trachomatis in urogenital specimens.评估Vidas衣原体检测法在泌尿生殖系统标本中检测和验证沙眼衣原体的能力。
J Clin Microbiol. 1997 Aug;35(8):2102-6. doi: 10.1128/jcm.35.8.2102-2106.1997.
3
Current methods of laboratory diagnosis of Chlamydia trachomatis infections.
沙眼衣原体感染的实验室诊断现行方法。
Clin Microbiol Rev. 1997 Jan;10(1):160-84. doi: 10.1128/CMR.10.1.160.
4
Use of PCR and direct immunofluorescence microscopy for confirmation of results obtained by Syva MicroTrak Chlamydia enzyme immunoassay.使用聚合酶链反应（PCR）和直接免疫荧光显微镜检查来确认由Syva MicroTrak衣原体酶免疫测定法获得的结果。
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5
Detection of Chlamydia trachomatis antigen by enzyme immunoassay: importance of confirmatory testing.通过酶免疫测定法检测沙眼衣原体抗原：确证试验的重要性
J Clin Pathol. 1995 Mar;48(3):214-7. doi: 10.1136/jcp.48.3.214.