Cross C E, O'Neill C A, Reznick A Z, Hu M L, Marcocci L, Packer L, Frei B
Division of Pulmonary and Critical Care Medicine, University of California, Davis 95616.
Ann N Y Acad Sci. 1993 May 28;686:72-89; discussion 89-90. doi: 10.1111/j.1749-6632.1993.tb39157.x.
In vitro exposure of fresh human plasma to cigarette smoke (CS) was used as a model for reactions that could be occurring in CS-exposed respiratory tract lining fluids (RTLFs) and lung parenchyma. The central focus of this model was to characterize the consumption of endogenous plasma antioxidants in relationship to the appearance of oxidized proteins and lipids as a consequence of exposure to CS, or to aldehydes present in CS. The amelioration of CS-induced protein and lipid oxidation in plasma by the addition of selective exogenous antioxidants was also assessed. We found that: (i) exposure of human plasma to gas phase CS causes both lipid peroxidation and protein oxidation, and endogenous ascorbic acid protects against lipid, but not protein, oxidation; (ii) whole CS causes protein oxidation, but does not induce lipid peroxidation; (iii) addition to plasma of aldehydes known to be present in CS causes protein damage, but does not induce either lipid peroxidation or oxidation of ascorbic acid; and (iv) exogenously added dihydrolipoic acid (DHLA) preserves ascorbic acid levels in plasma exposed to the gas phase of CS, and protects, to some extent, against lipid peroxidation; DHLA also protects against protein oxidation, whereas added glutathione (GSH) only protects against protein, but not lipid, oxidation.
将新鲜人体血浆在体外暴露于香烟烟雾（CS），以此作为暴露于CS的呼吸道内衬液（RTLFs）和肺实质中可能发生反应的模型。该模型的核心重点是表征内源性血浆抗氧化剂的消耗与因暴露于CS或CS中存在的醛类而导致的氧化蛋白质和脂质出现之间的关系。还评估了添加选择性外源性抗氧化剂对CS诱导的血浆蛋白质和脂质氧化的改善作用。我们发现：（i）人体血浆暴露于气相CS会导致脂质过氧化和蛋白质氧化，内源性抗坏血酸可防止脂质而非蛋白质氧化；（ii）完整的CS会导致蛋白质氧化，但不会诱导脂质过氧化；（iii）向血浆中添加已知存在于CS中的醛类会导致蛋白质损伤，但不会诱导脂质过氧化或抗坏血酸氧化；（iv）外源添加的二氢硫辛酸（DHLA）可维持暴露于CS气相的血浆中的抗坏血酸水平，并在一定程度上防止脂质过氧化；DHLA还可防止蛋白质氧化，而添加的谷胱甘肽（GSH）仅能防止蛋白质而非脂质氧化。
