在无血清体系中，成纤维细胞对乳腺癌细胞生长的刺激作用。
Fibroblast stimulation of breast cancer cell growth in a serum-free system.
作者信息
Ryan M C, Orr D J, Horgan K
机构信息
Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff, UK.
出版信息
Br J Cancer. 1993 Jun;67(6):1268-73. doi: 10.1038/bjc.1993.237.
Abstract
Conditioned media from 14 short term fibroblast cell lines were mitogenic for human breast cancer cells with different steroid receptor profiles in serum-free culture. Fibroblast-conditioned medium stimulated tritiated thymidine incorporation in short term culture and growth in a longer proliferation study as measured by the MTT colorimetric assay. Conditioned media from benign and malignant epithelial cells were non-stimulatory for breast cancer cells but that derived from endothelial cells showed similar stimulation to fibroblasts. Partial purification of fibroblast-conditioned medium identified a peptide with a molecular weight of approximately 8 kDa that showed no affinity for heparin and was mitogenic for MCF-7 breast cancer cells.
摘要
来自14种短期成纤维细胞系的条件培养基在无血清培养中对具有不同类固醇受体谱的人乳腺癌细胞有促有丝分裂作用。成纤维细胞条件培养基在短期培养中刺激了氚标记胸腺嘧啶核苷的掺入，并在一项更长时间的增殖研究中通过MTT比色法测定促进了细胞生长。良性和恶性上皮细胞的条件培养基对乳腺癌细胞无刺激作用，但内皮细胞来源的条件培养基对乳腺癌细胞的刺激作用与成纤维细胞相似。对成纤维细胞条件培养基进行部分纯化，鉴定出一种分子量约为8 kDa的肽，该肽对肝素无亲和力，对MCF-7乳腺癌细胞有促有丝分裂作用。
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本文引用的文献
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