Sueldo C E, Oehninger S, Subias E, Mahony M, Alexander N J, Burkman L J, Acosta A A
Department of Obstetrics and Gynecology, University of California San Francisco-Fresno 93703.
Fertil Steril. 1993 Jul;60(1):137-40. doi: 10.1016/s0015-0282(16)56051-8.
To determine if sperm exposure to P produces an enhancement in its fertilizing capacity.
Sperm from fertile donors were exposed to P at 0.1 and 1.0 microgram/mL for 1 or 24 hours. The effects on hyperactivated (HA) motility at 1 and 4 hours, acrosome reaction (as determined by Pisum sativum agglutinin or T6/antibody techniques), on human zona pellucida binding (by using the hemizona assay), and on the penetrating ability (by using the zona-free hamster ova assay) were evaluated.
Exposure to P at 1.0 microgram/mL enhanced HA motility after 1 and 4 hours of P exposure, the acrosome reaction after 24 hours' incubation, the number of sperm bound/hemizona after 1-hour incubation, and the penetration rates in the hamster ova assay at both incubation intervals.
Sperm exposure to P enhances its fertilizing capacity in fertile men, and further investigation is warranted as a possible treatment for male factor patients.
确定精子暴露于P是否会增强其受精能力。
将来自生育能力正常供体的精子分别暴露于浓度为0.1微克/毫升和1.0微克/毫升的P中1小时或24小时。评估其对1小时和4小时时的超活化（HA）运动能力、顶体反应（通过豌豆凝集素或T6/抗体技术测定）、与人透明带结合能力（通过半透明带试验）以及穿透能力（通过无透明带仓鼠卵试验）的影响。
暴露于1.0微克/毫升的P后，在P暴露1小时和4小时后HA运动能力增强，孵育24小时后顶体反应增强，孵育1小时后每个半透明带结合的精子数量增加，并且在两个孵育时间间隔的仓鼠卵试验中的穿透率均提高。
精子暴露于P可增强生育能力正常男性的受精能力，作为男性因素患者的一种可能治疗方法，值得进一步研究。
