遗传证据表明，酿酒酵母HIS4基因座处的减数分裂重组热点并不代表对称处理双链断裂的位点。
Genetic evidence that the meiotic recombination hotspot at the HIS4 locus of Saccharomyces cerevisiae does not represent a site for a symmetrically processed double-strand break.
作者信息
Porter S E, White M A, Petes T D
机构信息
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
出版信息
Genetics. 1993 May;134(1):5-19. doi: 10.1093/genetics/134.1.5.
Abstract
In the yeast Saccharomyces cerevisiae, the binding of the Rap1 protein to a site located between the 5' end of the HIS4 gene and the 3' end of BIK1 stimulates meiotic recombination at both flanking loci. By using strains that contain mutations located in HIS4 and BIK1, we found that most recombination events stimulated by the binding of Rap1 involve HIS4 or BIK1, rather than bidirectional events including both loci. The patterns of aberrant segregation indicate that most of the Rap1-stimulated recombination events do not represent the symmetric processing of a double-strand DNA break located at the Rap1-binding site.
摘要
在酿酒酵母中，Rap1蛋白与位于HIS4基因5'端和BIK1基因3'端之间的位点结合，可刺激两侧基因座的减数分裂重组。通过使用在HIS4和BIK1中含有突变的菌株，我们发现，由Rap1结合刺激的大多数重组事件涉及HIS4或BIK1，而不是包括两个基因座的双向事件。异常分离模式表明，大多数由Rap1刺激的重组事件并不代表位于Rap1结合位点的双链DNA断裂的对称加工。
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