White M A, Wierdl M, Detloff P, Petes T D
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9755-9. doi: 10.1073/pnas.88.21.9755.
In the yeast Saccharomyces cerevisiae, as in other eukaryotes, some regions of the genome have a much higher rate of meiotic recombination than others. We show below that the binding of the RAP1 protein to a site upstream of the HIS4 gene is necessary for a high rate of meiotic (but not mitotic) recombination at this locus. A mutation in the RAP1 binding site at HIS4 results in a decrease in recombination; overproduction of RAP1 causes an increase in recombination at HIS4 above wild-type levels.
与其他真核生物一样，在酿酒酵母中，基因组的某些区域减数分裂重组率比其他区域高得多。我们在下文表明，RAP1蛋白与HIS4基因上游位点的结合对于该位点高频率的减数分裂（而非有丝分裂）重组是必需的。HIS4处RAP1结合位点的突变导致重组减少；RAP1的过量表达使HIS4处的重组增加至高于野生型水平。
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