Lee Hwan Young, Kim Na Young, Park Myung Jin, Sim Jeong Eun, Yang Woo Ick, Shin Kyoung-Jin
Department of Forensic Medicine and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Korea.
J Forensic Sci. 2010 Nov;55(6):1422-9. doi: 10.1111/j.1556-4029.2010.01411.x.
The identification of missing casualties of the Korean War (1950-1953) has been performed using mitochondrial DNA (mtDNA) profiles, but recent advances in DNA extraction techniques and approaches using smaller amplicons have significantly increased the possibility of obtaining DNA profiles from highly degraded skeletal remains. Therefore, 21 skeletal remains of Korean War victims and 24 samples from biological relatives of the supposed victims were selected based on circumstantial evidence and/or mtDNA-matching results and were analyzed to confirm the alleged relationship. Cumulative likelihood ratios were obtained from autosomal short tandem repeat, Y-chromosomal STR, and mtDNA-genotyping results, and mainly confirmed the alleged relationship with values over 10⁵. The present analysis emphasizes the value of mini- and Y-STR systems as well as an efficient DNA extraction method in DNA testing for the identification of old skeletal remains.
朝鲜战争（1950 - 1953年）失踪人员身份鉴定一直采用线粒体DNA（mtDNA）图谱进行，但DNA提取技术以及使用更小扩增子方法的最新进展显著提高了从高度降解的骨骼遗骸中获取DNA图谱的可能性。因此，根据间接证据和/或mtDNA匹配结果，选取了21具朝鲜战争受害者的骨骼遗骸以及24份疑似受害者生物学亲属的样本，并进行分析以确认所声称的亲属关系。从常染色体短串联重复序列、Y染色体STR和mtDNA基因分型结果中获得累积似然比，主要通过大于10⁵的值确认了所声称的亲属关系。本分析强调了微型和Y-STR系统以及高效DNA提取方法在鉴定陈旧骨骼遗骸的DNA检测中的价值。
