Munshi A, Mehrotra R, Panda S K
Department of Pathology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi.
J Med Virol. 1993 May;40(1):53-8. doi: 10.1002/jmv.1890400111.
The therapeutic potential of plant extracts of Phyllanthus amarus and Phyllanthus maderas patensis for postexposure prophylaxis against infection by Hepadnaviruses was studied in ducklings infected by the duck hepatitis B virus (DHBV). Forty-four Pekin ducklings were inoculated intraperitoneally with DHBV at 24 hr post-hatch. They were treated by intraperitoneal injection of Phyllanthus amarus (aqueous extract) (100 mg/kg body weight) or Phyllanthus mad eraspatensis (alcoholic extract) (100 mg/kg body weight) for a period of 4 weeks. Infected ducklings treated with saline served as controls. Weekly serum samples obtained before, during, and after treatment were analysed for the presence of DHBV DNA in serum by dot blot hybridisation using alpha 32P-labelled probes. Liver tissue was collected after killing the ducks at various time intervals and was studied for replicative status of the viral DNA and liver histopathology; 17 of 21 ducks were viraemic on completion of treatment with Phyllanthus amarus. At 16 week posttreatment follow-up four of seven animals remained viraemic. Similar results were obtained with Phyllanthus maderaspatensis. There was no alteration in DHBV replication in the liver. No toxicity was observed with this treatment. These observations suggest that Phyllanthus amarus and Phyllanthus maderaspatensis are not useful as therapeutic agents for postexposure prophylaxis against DHBV infection.
在感染鸭乙型肝炎病毒（DHBV）的雏鸭中，研究了苦味叶下珠和马德拉叶下珠植物提取物对暴露后预防嗜肝DNA病毒感染的治疗潜力。44只北京雏鸭在孵化后24小时腹腔接种DHBV。它们通过腹腔注射苦味叶下珠（水提取物）（100毫克/千克体重）或马德拉叶下珠（醇提取物）（100毫克/千克体重）进行治疗，为期4周。用生理盐水处理的感染雏鸭作为对照。使用α-32P标记的探针通过斑点印迹杂交分析在治疗前、治疗期间和治疗后每周获得的血清样本中血清中DHBV DNA的存在情况。在不同时间间隔处死鸭子后收集肝脏组织，并研究病毒DNA的复制状态和肝脏组织病理学；在用苦味叶下珠治疗结束时，21只鸭子中有17只出现病毒血症。在治疗后16周的随访中，7只动物中有4只仍有病毒血症。马德拉叶下珠也得到了类似的结果。肝脏中DHBV复制没有改变。该治疗未观察到毒性。这些观察结果表明，苦味叶下珠和马德拉叶下珠作为暴露后预防DHBV感染的治疗药物并无用处。
