Leung P Y, Miyashita K, Young M, Tsao C S
Linus Pauling Institute of Science and Medicine, Palo Alto, CA 94306.
Anticancer Res. 1993 Mar-Apr;13(2):475-80.
The effect of L-ascorbate and its derivatives on the growth of malignant and nonmalignant cell lines has been examined. L-Ascorbate and its oxidative product dehydroascorbate were cytotoxic or lethal to the fast-growing malignant cells, but they were less toxic to nonmalignant cells. Two isomers of ascorbate, D-ascorbate and D-isoascorbate, both with 5% of the antiscorbutic potency and very high turnover rate, had similar activities. The cytotoxic effect of ascorbate was apparently not related to the metabolic or vitamin activities of ascorbate at the cellular level. Furthermore, studies on the viability of treated cells indicated that the observed effect on cell growth was not cytostatic in nature but was the result of a direct cell-killing action of ascorbate. Several groups of ascorbate derivatives were also tested; many of them were toxic to these cells. The results support the hypothesis that the cytotoxic activity of ascorbate was due to its chemical properties and that certain oxidation and degradation products of ascorbate were cytotoxic agents.
已研究了L-抗坏血酸及其衍生物对恶性和非恶性细胞系生长的影响。L-抗坏血酸及其氧化产物脱氢抗坏血酸对快速生长的恶性细胞具有细胞毒性或致死性，但对非恶性细胞的毒性较小。抗坏血酸的两种异构体，D-抗坏血酸和D-异抗坏血酸，抗坏血病效力均为5%且周转率非常高，具有相似的活性。抗坏血酸的细胞毒性作用显然与细胞水平上抗坏血酸的代谢或维生素活性无关。此外，对处理后细胞活力的研究表明，观察到的对细胞生长的影响本质上不是细胞抑制作用，而是抗坏血酸直接细胞杀伤作用的结果。还测试了几组抗坏血酸衍生物；其中许多对这些细胞有毒性。这些结果支持了以下假设：抗坏血酸的细胞毒性活性归因于其化学性质，并且抗坏血酸的某些氧化和降解产物是细胞毒性剂。
