Kobayashi M, Tanaka J, Imamura M, Maeda S, Iwasaki H, Tanaka M, Tsudu Y, Sakurada K, Miyazaki T
Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
Br J Haematol. 1993 Apr;83(4):535-8. doi: 10.1111/j.1365-2141.1993.tb04687.x.
In this report we show that IL-3 up-regulates the expression of IL-6-receptors on a plasma cell leukaemia cell line which proliferates in response to both IL-3 and IL-6. Quantitative binding studies and Scatchard analysis revealed that HSM-2.3 has a single class of high-affinity IL-6 binding sites (200/cell, dissociation constant at the equilibrium 1.43 x 10(-11) M). After stimulation with IL-3, HSM-2.3 has 1200 binding sites. IL-6 receptor mRNA expression was detected in HSM-2.3 only after stimulation with IL-3.
在本报告中，我们表明白细胞介素-3（IL-3）上调了一种浆细胞白血病细胞系上白细胞介素-6受体（IL-6R）的表达，该细胞系对IL-3和IL-6均有增殖反应。定量结合研究和Scatchard分析显示，HSM-2.3具有一类高亲和力的IL-6结合位点（200个/细胞，平衡解离常数为1.43×10⁻¹¹M）。用IL-3刺激后，HSM-2.3有1200个结合位点。仅在IL-3刺激后，才在HSM-2.3中检测到IL-6R mRNA表达。
