Reddy A, Sood A, Rust P F, Busby J E, Varn E, Mathur R S, Mathur S
Department of Obstetrics and Gynecology, College of Medicine, Medical University of South Carolina, Charleston 29425, USA.
J Assist Reprod Genet. 1995 Mar;12(3):217-23. doi: 10.1007/BF02211802.
PURPOSE AND STUDY PLAN: Men who are habitual smokers tend to have poor semen quality. We studied the effect of nicotine on sperm motility in vitro. Spermatozoa from 13 normal fertile nonsmoking donors, washed free of seminal plasma, were treated with medium alone (control); and, 10 mM, 5 mM, 1 mM and 0.1 mM nicotine (concentrations estimated to approximate residual concentrations of nicotine in the testes of heavy smokers). Computerized sperm motion analysis (CASA) was done at 2, 4, 6 and 24 h after incubation.
Sperm motility, beat/cross frequency, linearity and maximum anterior lateral head displacement (ALH max.) were significantly decreased across nominal dosages (P < 0.001 by repeated measures analysis of variance). Of the concentrations tested, 0.1 mM had no effect; 1 mM significantly decreased sperm motility (P = 0.003) and linearity (P = 0.02); 5 mM decreased the beat frequency (P = 0.001) and linearity (0.02); and 10 mM markedly decreased motility (P = 0.0001), beat frequency (P = 0.0002), linearity (P = 0.02) and ALH max. (P = 0.02). The interactions between dose and time were insignificant.
Nicotine at concentrations of > or = 1 mM significantly decreased sperm motion characteristics after different periods of incubation, whereas 0.1 mM concentration had the least effect.
目的和研究计划：习惯性吸烟的男性往往精液质量较差。我们在体外研究了尼古丁对精子活力的影响。从13名正常可育的非吸烟供体中获取精子，洗去精浆后，分别用单独的培养基（对照）；以及10 mM、5 mM、1 mM和0.1 mM尼古丁（这些浓度估计接近重度吸烟者睾丸中尼古丁的残留浓度）处理。孵育2、4、6和24小时后进行计算机辅助精子运动分析（CASA）。
在不同标称剂量下，精子活力、摆动/交叉频率、直线性和最大前外侧头部位移（最大ALH）均显著降低（重复测量方差分析，P < 0.001）。在所测试的浓度中，0.1 mM没有影响；1 mM显著降低精子活力（P = 0.003）和直线性（P = 0.02）；5 mM降低摆动频率（P = 0.001）和直线性（0.02）；10 mM显著降低活力（P = 0.0001）、摆动频率（P = 0.0002）、直线性（P = 0.02）和最大ALH（P = 0.02）。剂量和时间之间的相互作用不显著。
浓度≥1 mM的尼古丁在不同孵育时间后显著降低精子运动特征，而0.1 mM浓度的影响最小。
