Dias R S, Bambirra E A, Silva M E, Nicoli J R
Serviçio de Microbiologia de Alimentos, Fundação Ezequiel Dias, Belo Horizonte, MG, Brasil.
Braz J Med Biol Res. 1995 Mar;28(3):323-5.
The effect of orogastric administration of Saccharomyces boulardii on the anatomopathological aspect of the jejunal villi was studied in male Fischer rats (weighing about 40 g) orogastrically infected with a culture of Vibrio cholerae. Experimental and control groups received lyophilized S. boulardii (25 mg suspended in 0.5 ml saline) or 0.5 ml saline, respectively, three times a day for 10 days by gastric intubation. On day 5 of treatment, 0.5 ml of a culture of V. cholerae containing 10(8) viable cells was inoculated by gastric intubation into both groups. Histopathological examination of the jejunal mucosa showed extensive lesions of the superficial epithelium of the villi from the control group whereas few lesions of this superficial epithelium were observed in the experimental group. These data show that the inhibition of the action of the cholera toxin on enterocytes by S. boulardii suggested by recent results in vitro can be demonstrated in vivo.
在经口胃内接种霍乱弧菌培养物的雄性Fischer大鼠（体重约40克）中，研究了经口胃给予布拉酵母菌对空肠绒毛解剖病理学方面的影响。实验组和对照组分别通过胃插管每天三次、连续10天给予冻干的布拉酵母菌（25毫克悬浮于0.5毫升盐水中）或0.5毫升盐水。在治疗第5天，两组均通过胃插管接种0.5毫升含有10⁸个活细胞的霍乱弧菌培养物。空肠黏膜的组织病理学检查显示，对照组绒毛表面上皮有广泛病变，而实验组该表面上皮仅有少量病变。这些数据表明，近期体外实验结果所提示的布拉酵母菌对霍乱毒素作用于肠上皮细胞的抑制作用在体内也能得到证实。
