Gutnikov G
Department of Chemistry, California State Polytechnic University, Pomona 91768, USA.
J Chromatogr B Biomed Appl. 1995 Sep 15;671(1-2):71-89. doi: 10.1016/0378-4347(95)00116-z.
Most lipids are best characterized by their fatty acids which differ in chain length, the degree of unsaturation, configuration and position of the double bonds, and the presence of other functionalities. The fatty acid profiles are currently most frequently determined by capillary gas chromatography of the methyl esters which are prepared by a variety of methods. These are discussed with an emphasis on more recent developments, along with the stationary phases used for the separations and the methods employed for identification of the fatty acids. HPLC is applied less frequently for ascertaining fatty acid profiles than GC, but a very large number of derivatives for ultraviolet and fluorescence detection have been proposed. This method continues to evoke increasing interest, particularly in conjunction with fluorescence detection. This technique enables attainment of greater sensitivities than with standard GC methods employing flame ionization detection. Extensive applications of it to the analysis of free fatty acids in blood and other biomedical samples are clearly discernible. Other methods, including supercritical fluid chromatography, have found only limited application for fatty acid profiling.
大多数脂质的最佳特征在于其脂肪酸，这些脂肪酸在链长、不饱和度、双键的构型和位置以及其他官能团的存在方面存在差异。目前，脂肪酸谱最常通过多种方法制备的甲酯的毛细管气相色谱法来测定。本文将对这些方法进行讨论，重点关注最新进展，以及用于分离的固定相和用于脂肪酸鉴定的方法。与气相色谱法相比，高效液相色谱法较少用于确定脂肪酸谱，但已经提出了大量用于紫外和荧光检测的衍生物。这种方法继续引起越来越多的关注，特别是与荧光检测相结合时。与采用火焰离子化检测的标准气相色谱法相比，这种技术能够实现更高的灵敏度。它在血液和其他生物医学样品中游离脂肪酸分析的广泛应用清晰可见。其他方法，包括超临界流体色谱法，在脂肪酸谱分析中的应用非常有限。
