特定的乙型肝炎病毒负链DNA合成仅需要5'包装信号和3'近端直接重复序列DR1。
Specific hepatitis B virus minus-strand DNA synthesis requires only the 5' encapsidation signal and the 3'-proximal direct repeat DR1.
作者信息
Rieger A, Nassal M
机构信息
Zentrum für Molekulare Biologie, Universität Heidelberg, Germany.
出版信息
J Virol. 1996 Jan;70(1):585-9. doi: 10.1128/JVI.70.1.585-589.1996.
Abstract
Human hepatitis B virus (HBV) is a small DNA virus that replicates inside the viral nucleocapsid by reverse transcription of an RNA intermediate, the pregenome. The sequences encompassing the encapsidation signal epsilon and the direct repeat DR1 are present in two copies of this terminally redundant transcript. We have recently shown that HBV minus-strand DNA synthesis involves transfer of a short DNA primer copied from 5'-epsilon to 3'-DR1 (DR1*). Using transfection of HBV genomes with lesions in 3'-epsilon, and 5'-DR1 and its preceding sequence, we tested whether these additional elements contribute to the specificity of the transfer reaction. However, while some mutations affected proper plus-strand DNA formation, 5'-epsilon and DR1* were completely sufficient for correct minus-strand DNA production.
摘要
人类乙型肝炎病毒（HBV）是一种小型DNA病毒，它通过RNA中间体前基因组的逆转录在病毒核衣壳内进行复制。包含包装信号ε和直接重复序列DR1的序列存在于这个末端冗余转录本的两个拷贝中。我们最近发现，HBV负链DNA合成涉及从5'-ε复制的短DNA引物转移到3'-DR1（DR1*）。通过转染在3'-ε、5'-DR1及其上游序列存在损伤的HBV基因组，我们测试了这些额外元件是否有助于转移反应的特异性。然而，虽然一些突变影响了正链DNA的正常形成，但5'-ε和DR1*对于正确产生负链DNA是完全足够的。
相似文献
1
Specific hepatitis B virus minus-strand DNA synthesis requires only the 5' encapsidation signal and the 3'-proximal direct repeat DR1.特定的乙型肝炎病毒负链DNA合成仅需要5'包装信号和3'近端直接重复序列DR1。
J Virol. 1996 Jan;70(1):585-9. doi: 10.1128/JVI.70.1.585-589.1996.
2
A bulged region of the hepatitis B virus RNA encapsidation signal contains the replication origin for discontinuous first-strand DNA synthesis.乙肝病毒RNA衣壳化信号的一个凸起区域包含不连续的第一链DNA合成的复制起点。
J Virol. 1996 May;70(5):2764-73. doi: 10.1128/JVI.70.5.2764-2773.1996.
3
A pregenomic RNA sequence adjacent to DR1 and complementary to epsilon influences hepatitis B virus replication efficiency.与DR1相邻且与ε互补的前基因组RNA序列影响乙型肝炎病毒的复制效率。
Virology. 2002 Nov 10;303(1):199-210. doi: 10.1006/viro.2002.1645.
4
Initiation and termination of duck hepatitis B virus DNA synthesis during virus maturation.鸭乙型肝炎病毒DNA合成在病毒成熟过程中的起始与终止
J Virol. 1987 Dec;61(12):3832-40. doi: 10.1128/JVI.61.12.3832-3840.1987.
5
Hepadnavirus reverse transcription initiates within the stem-loop of the RNA packaging signal and employs a novel strand transfer.嗜肝DNA病毒的逆转录在RNA包装信号的茎环结构内起始，并采用一种新颖的链转移方式。
J Virol. 1994 Jun;68(6):3536-43. doi: 10.1128/JVI.68.6.3536-3543.1994.
6
Insertions within epsilon affect synthesis of minus-strand DNA before the template switch for duck hepatitis B virus.ε 内的插入影响鸭乙型肝炎病毒模板转换前负链 DNA 的合成。
J Virol. 1997 Jul;71(7):5345-54. doi: 10.1128/JVI.71.7.5345-5354.1997.
7
A novel cis-acting element facilitates minus-strand DNA synthesis during reverse transcription of the hepatitis B virus genome.一种新型顺式作用元件在乙型肝炎病毒基因组逆转录过程中促进负链DNA合成。
J Virol. 2004 Jun;78(12):6252-62. doi: 10.1128/JVI.78.12.6252-6262.2004.
8
Complementarity between epsilon and phi sequences in pregenomic RNA influences hepatitis B virus replication efficiency.前基因组RNA中ε序列和φ序列之间的互补性影响乙型肝炎病毒的复制效率。
Virology. 2007 Mar 15;359(2):371-81. doi: 10.1016/j.virol.2006.08.036. Epub 2006 Oct 23.
9
Base pairing between the 5' half of epsilon and a cis-acting sequence, phi, makes a contribution to the synthesis of minus-strand DNA for human hepatitis B virus.ε因子5'端的一半与顺式作用序列phi之间的碱基配对，对人类乙型肝炎病毒负链DNA的合成有贡献。
J Virol. 2006 May;80(9):4380-7. doi: 10.1128/JVI.80.9.4380-4387.2006.
10
RNA sequences controlling the initiation and transfer of duck hepatitis B virus minus-strand DNA.控制鸭乙型肝炎病毒负链DNA起始和转移的RNA序列。
J Virol. 1995 Jul;69(7):4283-91. doi: 10.1128/JVI.69.7.4283-4291.1995.
引用本文的文献
1
Hepatitis B Virus Nucleocapsid Assembly.乙型肝炎病毒核衣壳组装
J Mol Biol. 2025 Apr 30:169182. doi: 10.1016/j.jmb.2025.169182.
2
Hepatitis B Virus Epsilon (ε) RNA Element: Dynamic Regulator of Viral Replication and Attractive Therapeutic Target.乙型肝炎病毒 ε （ε） RNA 元件：病毒复制的动态调节剂和有吸引力的治疗靶点。
Viruses. 2023 Sep 12;15(9):1913. doi: 10.3390/v15091913.
3
Virtual Screening of Hepatitis B Virus Pre-Genomic RNA as a Novel Therapeutic Target.乙型肝炎病毒前基因组 RNA 的虚拟筛选作为一种新的治疗靶点。
Molecules. 2023 Feb 14;28(4):1803. doi: 10.3390/molecules28041803.
4
DNA Engineering and Hepatitis B Virus Replication.DNA工程与乙型肝炎病毒复制
Front Microbiol. 2021 Nov 11;12:783040. doi: 10.3389/fmicb.2021.783040. eCollection 2021.
5
Conservation of the HBV RNA element epsilon in nackednaviruses reveals ancient origin of protein-primed reverse transcription.乙肝病毒 RNA 元件 epsilon 在裸衣病毒中的保守性揭示了蛋白引物反转录的古老起源。
Proc Natl Acad Sci U S A. 2021 Mar 30;118(13). doi: 10.1073/pnas.2022373118.
6
Establishment of a system for finding inhibitors of ε RNA binding with the HBV polymerase.建立一个筛选与 HBV 聚合酶结合的 ε RNA 抑制剂的系统。
Genes Cells. 2020 Aug;25(8):523-537. doi: 10.1111/gtc.12778. Epub 2020 Jun 8.
7
A Tat-conjugated Peptide Nucleic Acid Tat-PNA-DR Inhibits Hepatitis B Virus Replication In Vitro and In Vivo by Targeting LTR Direct Repeats of HBV RNA.一种与反式激活转录蛋白（Tat）偶联的肽核酸Tat-PNA-DR通过靶向乙肝病毒（HBV）RNA的长末端重复序列（LTR）直接重复序列在体外和体内抑制HBV复制。
Mol Ther Nucleic Acids. 2016 Mar 15;5(3):e295. doi: 10.1038/mtna.2016.11.
8
Core protein: A pleiotropic keystone in the HBV lifecycle.核心蛋白：乙肝病毒生命周期中的一个多效性关键要素。
Antiviral Res. 2015 Sep;121:82-93. doi: 10.1016/j.antiviral.2015.06.020. Epub 2015 Jun 27.
9
Hepatitis B virus reverse transcriptase: diverse functions as classical and emerging targets for antiviral intervention.乙型肝炎病毒逆转录酶：作为抗病毒干预的经典和新出现靶点的多种功能
Emerg Microbes Infect. 2013 Sep;2(9):e56. doi: 10.1038/emi.2013.56. Epub 2013 Sep 4.
10
Molecular biology of the hepatitis B virus for clinicians.临床医生的乙型肝炎病毒分子生物学
J Clin Exp Hepatol. 2012 Dec;2(4):353-65. doi: 10.1016/j.jceh.2012.10.003. Epub 2012 Oct 18.
本文引用的文献
1
Hepatitis B virus replication.乙型肝炎病毒复制
Trends Microbiol. 1993 Sep;1(6):221-8. doi: 10.1016/0966-842x(93)90136-f.
2
Novel mechanism for reverse transcription in hepatitis B viruses.乙型肝炎病毒逆转录的新机制。
J Virol. 1993 Nov;67(11):6507-12. doi: 10.1128/JVI.67.11.6507-6512.1993.
3
The encapsidation signal on the hepatitis B virus RNA pregenome forms a stem-loop structure that is critical for its function.乙肝病毒RNA前基因组上的包装信号形成一个茎环结构，这对其功能至关重要。
Nucleic Acids Res. 1993 Aug 25;21(17):3967-75. doi: 10.1093/nar/21.17.3967.
4
An RNA stem-loop structure directs hepatitis B virus genomic RNA encapsidation.一种RNA茎环结构指导乙型肝炎病毒基因组RNA的衣壳化。
J Virol. 1993 Jun;67(6):3254-63. doi: 10.1128/JVI.67.6.3254-3263.1993.
5
Expression of functional hepatitis B virus polymerase in yeast reveals it to be the sole viral protein required for correct initiation of reverse transcription.功能性乙肝病毒聚合酶在酵母中的表达表明，它是正确启动逆转录所需的唯一病毒蛋白。
Proc Natl Acad Sci U S A. 1993 May 1;90(9):4107-11. doi: 10.1073/pnas.90.9.4107.
6
Cytoplasmic mRNA-protein interactions in eukaryotic gene expression.真核基因表达中的细胞质信使核糖核酸-蛋白质相互作用
Trends Biochem Sci. 1995 May;20(5):191-7. doi: 10.1016/s0968-0004(00)89006-4.
7
Role of RNA in enzymatic activity of the reverse transcriptase of hepatitis B viruses.RNA在乙型肝炎病毒逆转录酶酶活性中的作用。
J Virol. 1994 Dec;68(12):8437-42. doi: 10.1128/JVI.68.12.8437-8442.1994.
8
Site-specific RNA binding by a hepatitis B virus reverse transcriptase initiates two distinct reactions: RNA packaging and DNA synthesis.乙型肝炎病毒逆转录酶的位点特异性RNA结合引发两种不同反应：RNA包装和DNA合成。
J Virol. 1994 Sep;68(9):5579-87. doi: 10.1128/JVI.68.9.5579-5587.1994.
9
Two regions of an avian hepadnavirus RNA pregenome are required in cis for encapsidation.禽嗜肝DNA病毒RNA前基因组的两个区域在顺式作用下对衣壳化是必需的。
J Virol. 1994 Apr;68(4):2084-90. doi: 10.1128/JVI.68.4.2084-2090.1994.
10
Distinct requirements for primary sequence in the 5'- and 3'-part of a bulge in the hepatitis B virus RNA encapsidation signal revealed by a combined in vivo selection/in vitro amplification system.通过体内筛选/体外扩增联合系统揭示的乙型肝炎病毒RNA包装信号中凸起结构5'端和3'端对一级序列的不同要求。
Nucleic Acids Res. 1995 Oct 11;23(19):3909-15. doi: 10.1093/nar/23.19.3909.
Zotero 插件