Avila-Campos M J
Departamento de Microbiologia, Universidade de São Paulo, Brazil.
Rev Inst Med Trop Sao Paulo. 1995 May-Jun;37(3):215-7. doi: 10.1590/s0036-46651995000300006.
Haemolytic activity of sixty nine Actinobacillus actinomycetemcomitans strains on different animal and human blood types was examined by using a trypticase soy agar supplemented with yeast extract (0.5%). Blood types used were: rabbit, sheep and human (A, Rh+; A, Rh-; B, Rh+; B, Rh-; O, Rh+; O, Rh-; AB, Rh+; AB, Rh- groups). Plates were inoculated and, incubated in microaerophilic conditions, at 37 degrees C, for 48 h. The haemolytic activity of the tested strains was characterized as alpha-haemolysis. Only two isolates were not haemolytic on all blood types (2.9%), two strains were haemolytic only on human blood (one strain on AB, Rh+ group and another one on A, Rh+ and AB, Rh+ groups). No specificity between haemolysin produced by the tested strains and blood type was observed.
通过使用添加了0.5%酵母提取物的胰蛋白酶大豆琼脂，检测了69株伴放线放线杆菌菌株对不同动物和人类血型的溶血活性。所使用的血型包括：兔、绵羊和人类（A，Rh+；A，Rh-；B，Rh+；B，Rh-；O，Rh+；O，Rh-；AB，Rh+；AB，Rh-组）。接种平板，并在微需氧条件下于37℃孵育48小时。测试菌株的溶血活性被表征为α溶血。只有两株分离株对所有血型均无溶血活性（2.9%），两株菌株仅对人类血液有溶血活性（一株对AB，Rh+组有溶血活性，另一株对A，Rh+和AB，Rh+组有溶血活性）。未观察到测试菌株产生的溶血素与血型之间存在特异性。
