Sussman M D, Maes R K, Kruger J M, Spatz S J, Venta P J
Department of Microbiology, Michigan State University, East Lansing 44824, USA.
Virology. 1995 Dec 1;214(1):12-20. doi: 10.1006/viro.1995.9959.
Using a site-directed mutagenesis technique we constructed a new feline herpesvirus-1 recombinant strain containing a deletion in two genes encoding glycoproteins gI and gE. These proteins may have a role in virulence, the establishment of latency, and viral recurrence as shown in other herpesviruses of the varicella and simplex types. This recombinant was characterized and used to immunize juvenile cats against virulent virus challenge. Significant protection resulted from vaccination of cats by the subcutaneous route.
我们使用定点诱变技术构建了一种新的猫疱疹病毒1型重组毒株，该毒株在编码糖蛋白gI和gE的两个基因中存在缺失。正如在水痘和单纯疱疹病毒等其他疱疹病毒中所显示的那样，这些蛋白可能在毒力、潜伏的建立和病毒复发中发挥作用。对该重组毒株进行了特性鉴定，并用于免疫幼年猫以抵抗强毒病毒的攻击。通过皮下途径对猫进行疫苗接种产生了显著的保护作用。
