Sato F, Hagiwara Y, Kawase Y
Safety Evaluation Center, Central Research Laboratory, Chiba, Japan.
Arch Toxicol. 1995;69(7):444-9. doi: 10.1007/s002040050197.
Consumption of certain product lots of L-tryptophan (LT) has been reported to be epidemiologically associated with an outbreak of eosinophilia-myalgia syndrome (EMS) in the United States. Since the production lots were found to contain 3-phenylamino alanine (PAA) as an impurity, its effects were studied by administering the substance orally by gavage to 5-week-old Sprague-Dawley rats. Groups of animals were given PAA for 13 consecutive weeks at dose levels of 1, 10 and 100 mg/kg per day. The animals were killed at 4 or 8 weeks. Hematological and blood biochemical tests were performed and detailed histopathological observations were made. No significant abnormalities were observed in the test animals and in particular no EMS-like conditions. A brief summary of other animal studies using several species of rats and mice performed in our laboratory since 1989 on various LT related substances is also presented. No EMS-like effects were observed in these studies.
据报道，在美国，食用某些批次的L-色氨酸（LT）在流行病学上与嗜酸性粒细胞增多性肌痛综合征（EMS）的爆发有关。由于发现这些生产批次含有杂质3-苯基氨基丙氨酸（PAA），通过对5周龄的斯普拉格-道利大鼠经口灌胃给予该物质来研究其作用。将动物分组，每天按1、10和100mg/kg的剂量水平连续13周给予PAA。在4周或8周时处死动物。进行血液学和血液生化检测，并进行详细的组织病理学观察。在试验动物中未观察到明显异常，特别是没有类似EMS的情况。还简要总结了自1989年以来我们实验室使用几种大鼠和小鼠对各种LT相关物质进行的其他动物研究。在这些研究中未观察到类似EMS的效应。
