Kiyohara C, Hirohata T, Masuda Y
Department of Public Health, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Chemosphere. 1995 Oct;31(7):3673-80. doi: 10.1016/0045-6535(95)00216-u.
The separate and concurrent effects of polychlorinated dibenzo-p-dioxin (PCDD) and dibenzofuran (PCDF) congeners on aryl hydrocarbon hydroxylase (AHH) activity in human lymphoblastoid cells were examined. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-tetraCDD) induced AHH activity about 9-fold acetone-treated AHH activity, while octachlorodibenzo-p-dioxin (octaCDD) did not affect the AHH activity and 2,4,6,8-tetrachlorodibenzofuran (2,4,6,8-tetraCDF) reduced the AHH activity by 34%. The concurrent effect of 2,3,7,8-tetraCDD with 1,3,6,8-tetraCDD on AHH inducibility was much smaller than expected. The concurrent effect of 2,3,7,8-tetraCDD with 2,4,6,8-tetraCDF, octaCDD or 3,3',4,4',5,5'-hexaCB was as much as the effect of 2,3,7,8-tetraCDD alone. There was a difference between 3-methylcholanthrene (MC) and 2,3,7,8-tetraCDD in the concurrent effects of 3-methylsulfone-3',4,4',5-tetraCB (3-MSF-3',4,4',5-tetraCB) on AHH activity. The concurrent effect of MC with 3-MSF-3',4,4',5-tetraCB on AHH activity was equal to the effect of MC alone while that of 2,3,7,8-tetraCDD and 3-MSF-3',4,4',5-tetraCB on AHH activity was inhibitory. These results imply that congeners of PCDD and PCDF are considered to be more or less effective and isozymes of cytochrome P450, key enzymes for the AHH, induced by them are differently inhibited.
研究了多氯二苯并 - 对 - 二噁英（PCDD）和二苯并呋喃（PCDF）同系物对人淋巴母细胞中芳烃羟化酶（AHH）活性的单独和协同作用。2,3,7,8 - 四氯二苯并 - 对 - 二噁英（2,3,7,8 - 四氯二噁英）诱导的AHH活性约为丙酮处理的AHH活性的9倍，而八氯二苯并 - 对 - 二噁英（八氯二噁英）对AHH活性没有影响，2,4,6,8 - 四氯二苯并呋喃（2,4,6,8 - 四氯二苯并呋喃）使AHH活性降低了34%。2,3,7,8 - 四氯二苯并 - 对 - 二噁英与1,3,6,8 - 四氯二苯并 - 对 - 二噁英对AHH诱导性的协同作用远小于预期。2,3,7,8 - 四氯二苯并 - 对 - 二噁英与2,4,6,8 - 四氯二苯并呋喃、八氯二苯并 - 对 - 二噁英或3,3',4,4',5,5'-六氯联苯的协同作用与单独使用2,3,7,8 - 四氯二苯并 - 对 - 二噁英的作用相当。3 - 甲基胆蒽（MC）和2,3,7,8 - 四氯二苯并 - 对 - 二噁英在3 - 甲基砜 - 3',4,4',5 - 四氯联苯（3 - MSF - 3',4,4',5 - 四氯联苯）对AHH活性的协同作用方面存在差异。MC与3 - MSF - 3',4,4',5 - 四氯联苯对AHH活性的协同作用等于单独使用MC的作用，而2,3,7,8 - 四氯二苯并 - 对 - 二噁英与3 - MSF - 3',4,4',5 - 四氯联苯对AHH活性的作用具有抑制性。这些结果表明，PCDD和PCDF的同系物或多或少具有活性，由它们诱导的细胞色素P450同工酶（AHH的关键酶）受到不同程度的抑制。
