Berky J J, Zolotor L
In Vitro. 1977 Feb;13(2):63-75. doi: 10.1007/BF02615069.
Normal urinary bladder epithelial cells and cells derived from 2-acetylaminofluorene-induced urothelial carcinomas from male Balb/c mice were grown in monolayer culture and were characterized. Cell lines of normal bladder epithelium were mononucleated, sheet-forming cells, with a modal chromosome number of 40. Bladder epithelial carcinoma cells induced by 2-acetylaminofluorene were polynucleate, relatively fast growing, grew in soft agar, demonstrated a higher cloning efficiency than normal cells and formed tumors when inoculated into syngeneic hosts. Differences in morphology were recorded by photomicrography using phase optics and scanning electron microscopy.
将雄性Balb/c小鼠的正常膀胱上皮细胞以及由2-乙酰氨基芴诱导产生的膀胱上皮癌细胞进行单层培养并加以鉴定。正常膀胱上皮细胞系为单核、成片生长的细胞，众数染色体数为40。2-乙酰氨基芴诱导产生的膀胱上皮癌细胞为多核细胞，生长相对较快，能在软琼脂中生长，克隆效率高于正常细胞，接种到同基因宿主中会形成肿瘤。使用相差光学显微镜和扫描电子显微镜通过显微摄影记录形态学差异。
